Abstract
IN common with eukaryotic cellular mRNAs1–3, the terminal sequences in virus RNAs contain untranslated regions of variable length4–11. These regions are likely to contain binding sites for macromolecules involved in functions such as replication4 and translation4–6 of the RNA, and assembly of virus particles. We report here that, as a first step in identifying these sites we have sequenced between 33 and 156 nucleotides next to the 3′-terminal poly(A)12–14 of representative viruses from three of the four picornavirus genera15, the enteroviruses, cardioviruses and foot-and-mouth disease viruses (FMDV). The viruses chosen for these analyses allow us to make comparisons of the 3′-terminal sequences both within and between the three genera. Within each genus the 3′-terminal sequences are at least 60% homologous, whereas viruses from different genera show little similarity. The 3′-terminal sequences also show some unexpected features, including the lack of the putative signal sequence 5′-AAUAAA-3′ (ref. 1) in viruses from two genera.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Proudfoot, N. J., Cheng, C. C. & Brownlee, G. G. Prog. Nucleic Acid Res. molec. Biol. 19, 123–134 (1974).
Baralle, F. E. Cell 10, 549–558 (1977).
Ullrich, A. et al. Science 196, 1313–1319 (1977).
Zinder, N. D. RNA Phages (Cold Spring Harbor Laboratory, New York 1975).
Kozak, M. & Shatkin, A. J. J. molec. Biol. 112, 75–96 (1977).
Dasgupta, R., Shih, D. S., Saris, C. & Kaesberg, P. Nature 256, 624–628 (1975).
Richards, K., Guilley, H., Jonard, G. & Hirth, L. Eur. J. Biochem. 84, 513–519 (1978).
Briand, J-P., Jonard, G., Guilley, H., Richards, K. & Hirth, L. Eur. J. Biochem. 72, 453–463 (1977).
Pettersson, R. F., Flanegan, J. B., Rose, J. K. & Baltimore, D. Nature 268, 270–272 (1977).
Merregaert, J. et al. Eur. J. Biochem. 82, 55–63 (1978).
Haseltine, W., Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 989–993 (1977).
Frisby, D., Smith, J., Jeffers, V. & Porter, A. Nucleic Acid Res. 3, 2789–2810 (1976).
Yogo, Y. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 69, 1877–1882 (1972).
Chatterjee, N. K., Bachrach, H. L. & Polatnick, J. Virol. 69, 369–377 (1976).
Newman, J. F. E., Rowlands, D. J. & Brown, F. J. gen. Virol. 18, 171–180 (1973).
Proudfoot, N. J. J. molec. Biol. 107, 491–525 (1976).
Porter, A. G., Merregaert, J., van Emmelo, J. & Fiers, W. Eur. J. Biochem. 87, 551–561 (1978).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Sanger, F. & Coulson, A. R. J. molec. Biol. 94, 441–448 (1975).
Brownlee, G. G. & Cartwright, E. M. J. molec. Biol. 114, 93–117 (1977).
Weissmann, C., Billeter, M. A., Goodman, H. M., Hindley, J. & Weber, H. A. Rev. Biochem. 42, 303–328 (1973).
Fiers, W. et al. Nature 273, 113–120 (1978).
Seeburg, P. H., Shine, J., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 486–494 (1977).
Schwartz, D. E., Zamecnik, P. C. & Weith, H. L. Proc. natn. Acad. Sci. U.S.A. 74, 994–998 (1977).
Yogo, Y., & Wimmer, E. Biochem. biophys. Res. Commun. 61, 1101–1109 (1974).
Tinoco, I., Uhlenbeck, O. C. & Levine, M. D. Nature 230, 362–367 (1971).
Shine, J., Seeburg, P. H., Martial, J. A., Baxter, J. D. & Goodman, H. M. Nature 270, 494–499 (1977).
Wilson, J. T., de Riel, J. K., Forget, B. G., Marotta, C. A. & Weissman, S. M. Nucleic Acid Res. 4, 2353–2368 (1977).
Proudfoot, N. J. Cell 10, 559–570 (1977).
Subramanian, K. N., Dhar, R. & Weissman, S. M. J. biol. Chem. 252, 355–367 (1977).
Brown, F. et al. Nature 251, 342–344 (1974).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PORTER, A., FELLNER, P., BLACK, D. et al. 3′-Terminal nucleotide sequences in the genome RNA of picornaviruses. Nature 276, 298–301 (1978). https://doi.org/10.1038/276298a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/276298a0
This article is cited by
-
The structure of eight distinct cloned human leukocyte interferon cDNAs
Nature (1981)
-
Primary structure, gene organization and polypeptide expression of poliovirus RNA
Nature (1981)
-
Molecular cloning of foot and mouth disease virus genome and nucleotide sequences in the structural protein genes
Nature (1981)
-
The cDNA for the β-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3′-untranslated region
Nature (1980)
-
Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA
Nature (1979)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
