Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Identification of the haem ligands of cytochrome b562 by X-ray and NMR methods

Nature volume 275, pages 245–247 (1978)Cite this article

Abstract

X-RAY crystallography and nuclear magnetic resonance (NMR) are the two most powerful physical techniques for studying macromolecular structure. They are often used to complement one another in the study of a particular molecule. Crystallographic results have been of tremendous help in the NMR peak assignments which are subsequently used to obtain structural information in solution1,2. We report the combined use of both X-ray crystallography and NMR to identify MET-7 and HIS-106 as the two ligands of the haem iron of cytochrome b562.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Rich, A. Acc. Chem. Res. 10, 388–396 (1977).

    Article  CAS  Google Scholar 

  2. Reid, B. R. & Hurd, R. E. Acc. Chem. Res. 10, 396–402 (1977).

    Article  CAS  Google Scholar 

  3. Itagaki, E. & Hager, L. P. Biochem. biophys. Res. Commun. 32, 1013–1019 (1968).

    Article  CAS  Google Scholar 

  4. Itagaki, E. & Hager, L. P. J. biol. Chem. 241, 3687–3695 (1966).

    CAS  PubMed  Google Scholar 

  5. Warme, P. K. & Hager, L. P. Biochemistry 9, 4244–4251 (1970).

    Article  CAS  Google Scholar 

  6. Czerwinski, E. W., Mathews, F. S., Hollenberg, P., Drickamer, K. & Hager, L. P. J. molec. Biol 71, 819–821 (1972).

    Article  CAS  Google Scholar 

  7. Czerwinski, E. W. & Mathews, F. S. J. molec. Biol. 86, 49–57 (1974).

    Article  CAS  Google Scholar 

  8. Wuthrich, K. NMR in Biological Research: Peptides and Proteins (North-Holland, Amsterdam, 1976) (and refs therein).

    Google Scholar 

  9. Keller, R., Groudinsky, O. & Wuthrich, K. Biochim. biophys. Acta 427, 497–511 (1976).

    Article  CAS  Google Scholar 

  10. Dobson, C. M. et al. Nature 249, 425–429 (1974).

    Article  ADS  CAS  Google Scholar 

  11. McDonald, C. C., Phillips, W. D. & Le Gall, J. Biochemistry 13, 1952–1959 (1974).

    Article  CAS  Google Scholar 

  12. Moura, J. J. G. et al. FEBS Lett 81, 275–280 (1977).

    Article  CAS  Google Scholar 

  13. McDonald, C. C., Phillips, W. D. & Vinagradov, S. N. Biochem. biophys. Res. Commun. 3, 442–449 (1969).

    Article  Google Scholar 

  14. Wuthrich, K. Proc. natn. Acad. Sci. U.S.A. 63, 1971–1978 (1969).

    Article  Google Scholar 

  15. Keller, R. M., Wuthrich, K. & Schejter, A. Biochim. biophys. Acta 491, 409–415 (1975).

    Article  Google Scholar 

  16. Moore, G. R. & Williams, R. J. P. FEBS Lett. 79, 229–232 (1977).

    Article  CAS  Google Scholar 

  17. DerVartanian, D. V. & Le Gall, J. Biochim. biophys. Acta 346, 79–99 (1974).

    Article  CAS  Google Scholar 

  18. Mathews, F. S., Levine, M. & Argos, P. Nature new Biol. 233, 15–16 (1971).

    Article  ADS  CAS  Google Scholar 

  19. Campbell, I. D., Dobson, C. M., Jeminet, G. J. & Williams, R. J. P. FEBS Lett. 49, 115–118 (1974).

    Article  CAS  Google Scholar 

  20. Bleich, H. E. & Glasel, J. A. J. Mag. Res. 18, 401–405 (1975).

    ADS  CAS  Google Scholar 

  21. Bricogne, G. Acta Crystallogr. A 32, 832–847 (1976).

    Article  Google Scholar 

  22. Velick, S. F. and Strittmatter, P. J. biol. Chem. 221, 265–275 (1956).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centro Quimica Estrutural, IST, Lisboa, Portugal

    ANTONIO V. XAVIER

  2. Freshwater Biological Institute, University of Minnesota, Navarre, Minnesota, 55392

    ANTONIO V. XAVIER

  3. Department of Physiology and Biophysics, Washington University School of Medicine, St Louis, Missouri

    EDMUND W. CZERWINSKI, PAUL H. BETHGE & F. SCOTT MATHEWS

Authors
  1. ANTONIO V. XAVIER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. EDMUND W. CZERWINSKI
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. PAUL H. BETHGE
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. SCOTT MATHEWS
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

XAVIER, A., CZERWINSKI, E., BETHGE, P. et al. Identification of the haem ligands of cytochrome b562 by X-ray and NMR methods. Nature 275, 245–247 (1978). https://doi.org/10.1038/275245a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/275245a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing