Abstract
THE evidence of C-type virus expression in man includes electron microscopic observation of virus-like particles in 30–50% of a small number of human placentas1–4. Because of the limitations of ultrastructural surveys (sample number, specimen size, false negatives) we attempted to detect C-type virus expression in human placenta by indirect immunofluorescence using antisera raised against HEL-12 virus; this is a C-type retrovirus recovered from human embryonic lung cells after in vitro cultivation5. We demonstrate here C-type virus-related antigen in 24 of 24 term placentas. The specificity of the immunofluorescence reaction for viral antigen was confirmed by serum absorption tests with purified virus. Preliminary isolation of the reactive antigen from placenta yielded a glycoprotein-rich fraction (β-μ) which blocked binding of anti-HEL-12 virus antiserum with placenta. In addition, antisera raised against β-μ, reacted with HEL-12 virus-infected cells as well as with human placenta.
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SAWYER, M., NACHLAS, N. & PANEM, S. C-type viral antigen expression in human placenta. Nature 275, 62–64 (1978). https://doi.org/10.1038/275062a0
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DOI: https://doi.org/10.1038/275062a0
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