Letter | Published:

In vivo immunisation and reaction against TNP-modified splenocytes

Nature volume 274, pages 806808 (24 August 1978) | Download Citation

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Abstract

CYTOTOXICITY tests in vitro detect some components of the graft rejection reaction. In one such test, antigenically modified (hapten trinitrophenyl TNP) mouse lymphocytes are detected by the action of cytolytic T lymphocytes (CTL) if the sensitisation of the effector cells is carried out in vitro1. In the CTL test there is evidence2 that the reaction is not against the hapten, but against antigenic membrane groups coded by H–2D and H–2K genes, a phenomenon termed ‘H–2 restriction’. In contrast, immunisation in vivo with TNP-modified lymphocytes results in a low production of CTL as detected in vitro, due probably to production of T suppressor cells3. The aim of the present work was to compare the in vivo efficacy of the response of the TNP splenocytes with that obtained in vitro, and to determine whether the H–2 restriction applies. Our results show that the immunogenetic implications differed according to whether the test was carried out in vivo or in vitro, and suggest that the effectors for target cell destruction are also different.

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Author information

Affiliations

  1. INSERM–95 Research Unit, Plateau de Brabois, 54500 Vandoeuvre, France

    • P. BISCHOFF
    •  & D. OTH

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DOI

https://doi.org/10.1038/274806a0

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