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Molecular basis of base substitution hotspots in Escherichia coli

Nature volume 274, pages 775780 (24 August 1978) | Download Citation

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Abstract

In the lacI gene of Escherichia coli spontaneous base substitution hotspots occur at 5-methylcytosine residues. The hotspots disappear when the respective cytosines are not methylated. We suggest that the hotspots may result from the spontaneous deamination of 5-methylcytosine to thymine, which is not excised by the enzyme DNA-uracil glycosidase.

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Affiliations

  1. Département de Biologie Moléculaire, Université de Genève, Geneva, Switzerland

    • Christine Coulondre
    •  & Jeffrey H. Miller
  2. Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138

    • Philip J. Farabaugh
    •  & Walter Gilbert

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https://doi.org/10.1038/274775a0

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