Article | Published:

Revised sequence for the lac repressor

Nature volume 274, page 767 (24 August 1978) | Download Citation

Subjects

Abstract

THE elucidation of the DNA sequence of the lacI gene1 has indicated the existence of an additional 13 amino acids not reported in the original protein sequence2. Recently a direct protein chemical verification of the suggested additional 11 amino acids in the represser after position 147 and of 2 amino acids after residue 230 (formerly residue 2192) has been made. The strategy of the re-examination of the protein sequence included the isolation of [14C]carboxyamidomethylated cysteine-containing peptides which contain the sought-after amino acids and the automated Edman degradation3 of these peptides. Thus, a quantitative detection and sequence determination was possible of all fragments including insoluble peptides since there was the suspicion that solubility problems and semi-quantitative manual sequencing methods2 gave rise to the encountered discrepancies.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    Nature 274, 765–769 (1978).

  2. 2.

    , , & Proc. natn. Acad. Sci. U.S.A. 70, 3576–3580 (1973); , , , , & Eur. J. Biochem. 59, 491–509 (1975).

  3. 3.

    & Eur. J. Biochem. 1, 80–91 (1967).

  4. 4.

    , & J. biol. Chem. 245, 1895–1902 (1970).

  5. 5.

    & Proc. natn. Acad. Sci. U.S.A. 69, 3506–3509 (1972).

  6. 6.

    in Solid Phase Methods in Protein Sequence Analysis (eds Previero, A. & Coletti-Previero, M.-A.) (North-Holland, Amsterdam, in the press).

Download references

Author information

Affiliations

  1. Institut für Genetik der Universität zu Köln, Köln, FRG

    • KONRAD BEYREUTHER

Authors

  1. Search for KONRAD BEYREUTHER in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/274767a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.