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Revised sequence for the lac repressor

Nature volume 274, page 767 (24 August 1978) | Download Citation



THE elucidation of the DNA sequence of the lacI gene1 has indicated the existence of an additional 13 amino acids not reported in the original protein sequence2. Recently a direct protein chemical verification of the suggested additional 11 amino acids in the represser after position 147 and of 2 amino acids after residue 230 (formerly residue 2192) has been made. The strategy of the re-examination of the protein sequence included the isolation of [14C]carboxyamidomethylated cysteine-containing peptides which contain the sought-after amino acids and the automated Edman degradation3 of these peptides. Thus, a quantitative detection and sequence determination was possible of all fragments including insoluble peptides since there was the suspicion that solubility problems and semi-quantitative manual sequencing methods2 gave rise to the encountered discrepancies.

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  1. Institut für Genetik der Universität zu Köln, Köln, FRG



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