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DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation

Nature volume 274, pages 762765 (24 August 1978) | Download Citation

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Abstract

The promoter for a weakly expressed constitutive gene, the lactose repressor gene (lacI), has been sequenced, along with an ‘up’ promoter mutation Iq. The 10-fold enhancement in I expression found in Iq is the result of a single base change at position −35. To facilitate the sequencing, the lacI gene was cloned in a small plasmid.

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Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, Biological Laboratories, Cambridge, Massachusetts 02138

    • Michèle P. Calos

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https://doi.org/10.1038/274762a0

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