Abstract
CHLOROPLAST DNA (ctDNA) from Euglena gracilis contains 25–27 cistrons for tRNA1,2 and two cistrons each for 16S and 23S tRNA3, and 2.8% of the DNA codes for non-ribosomal RNA containing polyadenosine4. The tRNA from purified chloroplasts complements 18 cistrons, whereas whole cells contain species of tRNA complementing 7–9 additional cistrons1. Chromatography on DBAE-cellulose separates the tRNA into two fractions1. Uniquely modified bases are found in the fraction II tRNA. Isolated chloroplasts do not contain fraction II tRNA but this tRNA does hybridise to 7 or 8 cistrons on the ct DNA1. The present results demonstrate that fraction I and fraction II tRNAs are coded by separate and distinct cistrons and that cytoplasmic polyribosomes contain the fraction II tRNA. Further, the aminoacyl acceptor species in fraction II tRNA from whole cells and from cytoplasmic polyribosomes are the same, but the relative abundance of each species varies in the two samples.
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References
McCrea, J. M. & Hershberger, C. L. Nucleic Acids Res. 3, 2005–2018 (1976).
Swartzbach, S. D., Hecker, L. I. & Barnett, W. E. Proc. natn. Acad. Sci. U.S.A. 73, 1984–1988 (1976).
Mielenz, J. R., Milner, J. J. & Hershberger, C. L. J. Bact. 130, 860–868 (1977).
Milner, J. J., Hershberger, C. L. & Buetow, D. E. Eur. J. Biochem. (submitted).
McCutchen, T. F., Gilham, P. P. & Soll, D. Nucleic Acids Res. 2, 853–864 (1975).
Avadhani, N. G. & Buetow, D. E. Biochem. J. 128, 353–365 (1972).
Smith, D. W. E. Science 190, 529–535 (1975).
Burkard, G., Guillemaut, P., Steinmetz, A. & Weil, J. H. Biochem. Soc. Symp. 38, 43–56 (1973).
Burkard, G., Vaultier, J. P. & Weil, J. H. Phytochemistry 11, 1351–1353 (1973).
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McCREA, J., HERSHBERGER, C. Chloroplast DNA codes for tRNA from cytoplasmic polyribosomes. Nature 274, 717–719 (1978). https://doi.org/10.1038/274717a0
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DOI: https://doi.org/10.1038/274717a0
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