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Induction and separation of antigen-dependent T helper and T suppressor cells in man

Naturevolume 274pages586587 (1978) | Download Citation



INDUCTION of plaque-forming cells (PFC), specific for sheep red blood cells (SRBC) or ovalbumin (OA), from human peripheral blood requires the cooperation of surface IgM-positive B cells and E-rosette-forming T lymphocytes1,2. At high antigen concentrations PFC responses are suppressed2 and it seemed that this effect might be mediated in part by antigen-stimulated T lymphocytes2,3. We have studied the functional activity of purified T cells which had been preincubated (‘primed’) for 24 h in the presence of a high concentration of antigen. We report here that such primed cell preparations contain antigen-specific suppressor cells which interfere with the induction of PFC in autologous target PFC cultures. These suppressor cells and their precursors could be isolated in the theophylline-sensitive lymphocyte fraction4.

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  1. 1

    Dosch, H. M. & Gelfand, E. W. J. Immun. Meth. 11, 107 (1976).

  2. 2

    Dosch, H. M. & Gelfand, E. W. J. Immun. 118, 302 (1977).

  3. 3

    UytdeHaag, F., Heynen, C. J. & Ballieux, R. E. Nature 271, 556 (1978).

  4. 4

    Limatibul, S., Shore, A., Dosch, H. M. & Gelfand, E. W. Clin. exp. Immun. (in the press).

  5. 5

    Moretta, L., Webb, S., Grossi, C. E., Lydyard, P. M. & Cooper, M. D. J. exp. Med. 146, 184 (1977).

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  1. Department of Immunology, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada, M5G 1X8



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