Letter | Published:

Glycosylation is not necessary for membrane insertion and cleavage of Semliki Forest virus membrane proteins

Nature volume 274, pages 487490 (03 August 1978) | Download Citation

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Abstract

RECENT translation studies in vitro1,2,33 have shown that the synthesis of membrane ectoproteins, like the glycoproteins of vesicular stomatitis virus (VSV) and Semliki Forest virus (SFV), (budding, enveloped viruses), occurs simultaneously with their insertion into membranes, their glycosylation and their proteolytic processing. We have studied the synthesis of SFV membrane proteins in infected cells treated with tunicamycin, which prevents glycosylation of proteins by inhibiting the formation of the lipid bound sugar intermediates needed for protein glycosylation3–5. We have found that glycosylation is not a prerequisite for correct insertion and cleavage of such membrane ectoproteins.

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Author information

Affiliations

  1. European Molecular Biology Laboratory, Postfach 10 2209, D6900, Heidelberg, FRG

    • HENRIK GAROFF
  2. Institut für Virologie, Justus Liebig University, D6300 Giessen, FRG

    • RALPH T. SCHWARZ

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https://doi.org/10.1038/274487a0

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