Letter | Published:

An antiserum against 9,11-azo-15-hydroxy-prosta-5,13-dienoic acid recognises and binds prostaglandin endoperoxides

Abstract

THE prostaglandin endoperoxides PGG2 and PGH2, derived from arachidonic acid, are transient regulators in mammalian cells1–4. On formation, the endoperoxides are rapidly converted to PGF, PGE2, PGD2, and their 15-keto-PG metabolites; to 12-L-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and malonaldehyde5; to thromboxane A2 (refs 6, 7); and to PGI2 (prostacyclin or PGX; ref. 8). The enzymatic composition of each tissue governs the nature and extent of these conversions. By their direct influence and as precursors for other prostaglandins, the endoperoxides are primary cellular regulators; unfortunately, their short half life and intrinsic instability makes direct determination of PGH2 related phenomena difficult. By using as the hapten a stable mimic9, stereochemically similar to the physiologically authentic endoperoxides, we have prepared antibodies which recognise and bind PGG2 and PGH2. Enzymatic and chemical conversion of the endoperoxides and their pharmacological influence on platelet aggregation in vitro are retarded by these antibodies.

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