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Temperature-induced seizure and frequency-dependent neuromuscular block in a ts mutant of Drosophila


SINGLE-GENE conditional mutants of Drosophila melanogaster which affect both development and the physiology of the adult present a valuable opportunity for studying the effects of a specific biological lesion on both individual physiological processes, and on whole animal biology encompassing the growth and development of the organism. Temperature-sensitive mutations at the shibire locus of D. melanogaster produce both developmental and neurophysiological defects during various periods of the animal's life cycle, and thus illustrates how a single gene product can participate in both synaptic transmission in the adult as well as in the process of differentiation during development. The importance of analysing such a mutant lies in the hope of revealing a biological mechanism common to both adult physiology and the developmental process. Such a revelation might contribute insights into both processes. The work presented here is an analysis of the adult physiological phenotype of the shibire mutant.

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  1. 1

    Grigliatti, T. A., Hall, L., Rosenbluth, R. & Suzuki, D. T. Molec. gen. Genet. 120: 107–114 (1973).

  2. 2

    Poodry, C. A., Hall, L. & Suzuki, D. T. Devl Biol. 32, 373–386 (1973).

  3. 3

    Kelly, L. E. & Suzuki, D. T. Proc. natn. Acad. Sci. U.S.A. 71, 4906–4909 (1974).

  4. 4

    Ikeda, K., Ozawa, S. & Hagiwara, S. Nature 259, 489–491 (1976).

  5. 5

    Siddiqi, O. & Benzer, S. Proc. natn. Acad. Sci. U.S.A. 73, 3252–3257 (1976).

  6. 6

    Ikeda, K. & Kaplan, W. Proc. natn. Acad. Sci. U.S.A. 66, 765–772 (1970).

  7. 7

    Stark, W. S. & Wasserman, G. S. Dros. Info. Ser. (Research Notes) 49–63 (1972).

  8. 8

    Alawi, A. A. & Pak, W. L. Science 172, 1055–1057 (1971).

  9. 9

    Kuffler, S. W. & Nicholls, J. G. in From Neuron to Brain 287–288 (Sinauer, Massachusetts, 1976).

  10. 10

    Katz, B. & Miledi, R. J. Physiol., Lond. 192, 407–436 (1967).

  11. 11

    Orland, R. K., Nicholls, J. G. & Kuffler, S. W. J. Neurophysiol. 29, 788–806 (1966).

  12. 12

    Rutherford, D. T., Nastuk, W. L. & Gennaro, J. F. J. Cell Biol. 70, No. 2 part 2, 375a (1976).

  13. 13

    Heuser, J. C. & Reese, T. S. J. Cell Biol. 57: 315 (1973).

  14. 14

    Sypert, G. W. & Ward, A. A. Jr, Expl Neurol. 33, 239–255 (1971).

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