Letter | Published:

Ionic channel formation in a living cell membrane

Abstract

THERE have been several attempts to study cell membrane formation by the use of protoplasmic droplets extruded from plant cells into appropriate solutions1. The formation of a membrane on the surface of such droplets has been confirmed by optical, electrical and other measurements2–4, but some properties of these unnatural membranes have been questioned5. I report here experiments which indicate the possibility of observing membrane formation in a living cell. The method was based on the sealing effect6–8 which isolates a microelectrode tip from protoplasm into which it has been inserted.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Kamiya, N. & Kuroda, K. Proc. Japan Acad. 33, 149–152 (1957).

  2. 2

    Inoue, I., Ishima, Y., Horie, H. & Takenaka, T. Proc. Japan Acad. 47, 549–553 (1971).

  3. 3

    Inoue, I., Ishida, N. & Kobatake, Y. Biochim. biophys. Acta 330, 27–38 (1973); 367, 24–31 (1974).

  4. 4

    Koppenhofer, E. Pflugers Arch. ges. Physiol. 347, R36 (1974); 355, R82 (1975); 258, 179–187 (1975).

  5. 5

    Koppenhofer, E. Pflugers Arch. ges. Physiol. 361, 263–267 (1976).

  6. 6

    Umrath, K. Protoplasma 16, 173–188 (1932).

  7. 7

    Walker, N. A. Aust. J. biol. Sci. 8, 476–489 (1955).

  8. 8

    Skierczynska, J. J. exp. Bot. 19, 389–406 (1968).

  9. 9

    Moore, J. W. in Biophysics and Physiology of Excitable Membranes (ed. Adelman W. J.) 159 (Van Nostrand Reinhold, New York, 1971).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.