Letter | Published:

Solvent-depleted bilayer membranes from concentrated lipid solutions

Nature volume 272, pages 839840 (27 April 1978) | Download Citation

Subjects

Abstract

MODEL bimolecular lipid membranes (BLMs) contain lipid solvents, such as n-alkanes, which are not normally present in biological membranes1,2. Studies aimed at overcoming this limitation have established that the volume of solvent within the BLM can be estimated by measurement of the bilayer specific geometric capacitance3–6. We have used this aproach to evaluate the distribution of solvent between the BLM and its supporting organic bulk solution (torus). Decreasing the solvent content of the torus solution resulted in a decrease in the solvent content of the BLM. Moreover, membranes that are nearly free of residual solvent could be formed from highly concentrated lipid solutions having a minimal amount of solvent. The observed concentration dependence of BLM capacitance may reconcile the differences between membranes formed from submerged bulk solutions and those formed from excess lipid monolayers7.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , , & Nature 194, 979–980 (1962).

  2. 2.

    , & J. Membrane Biol. 10, 11–30 (1972).

  3. 3.

    , & J. Membrane Biol. 5, 277–296 (1971).

  4. 4.

    Biophys. J. 15, 95–117 (1975).

  5. 5.

    & Biochim. biophys. Acta 455, 721–738 (1976).

  6. 6.

    , & J. Colloid Interface Sci. 58, 26–35 (1977).

  7. 7.

    , & Biochim. biophys. Acta 394, 323–334 (1975).

  8. 8.

    & Proc. natn. Acad. Sci. U.S.A. 69, 3561–3566 (1972).

  9. 9.

    , & J. Membrane Biol. 1, 346–382 (1969).

  10. 10.

    Biophys. J. 10, 1127–1148 (1970).

  11. 11.

    , & Spec. Disc. Faraday Soc. 1, 46–56 (1970).

  12. 12.

    Biochim. biophys. Acta 323, 343–350 (1973).

  13. 13.

    , , & Biochim. biophys. Acta 470, 17–34 (1977).

Download references

Author information

Affiliations

  1. Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77550

    • RONALD WALDBILLIG
    •  & GABOR SZABO

Authors

  1. Search for RONALD WALDBILLIG in:

  2. Search for GABOR SZABO in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/272839a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.