Abstract
EXPERIMENTAL allergic neuritis (EAN), an autoimmune demye-linating disease of peripheral nerve, and a model of human inflammatory polyneuritis (Guillain–Barré syndrome), can be produced by immunisation with peripheral nerve tissue in complete Freund's adjuvant (CFA)1,2. EAN, like its central nervous system counterpart, experimental allergic encephalomyelitis (EAE), has been considered as the result of cell-mediated immunity2,3, which can be transferred with lymphoid cells4,5. Sera from animals with EAN or EAE induced by whole nervous tissue antigen are capable of demyelinating myelinated organotypic cultures of peripheral or central nervous system origin, respectively6,7. The demyelinating factor in EAE sera is immunoglobulin8,9. However, little evidence has been presented to suggest that the demyelinating antibodies which can be demonstrated in vitro are involved in the pathogenesis of these diseases in vivo4,10–12. EAN and EAE have not been transferred passively with serum from affected to recipient animals by systemic4,11 or intravitreous injection12. We report here the first successful transfer of demyelination with EAN serum. Antiserum was injected directly into nerve parenchyma of recipient animals to circumvent the blood–nerve barrier. The demyelination was associated with macrophages but not with lymphocytic infiltration. The ability of sera to transfer demyelination correlated with their capacity to demyelinate cultured dorsal root ganglia. Organ specificity and complement dependency strongly suggested that the reaction was antibody-dependent and complement-mediated.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Waksman, B. W. & Adams, R. D. J. exp. Med. 102, 213–236 (1955).
Arnason, B. G. in Peripheral Neuropathy (eds Dyck, P. J. Thomas, P. K. & Lambert, E. H.) 1110–1148 (Saunders, Philadelphia, 1975).
Behan, P. 0., Lamarche, J. B., Feldman, R. G. & Sheramata, W. A. Lancet i, 421–422 (1970).
Ästrom, K. E. & Waksman, B. H. J. Pathol. Bacteriol. 83, 89–106 (1962).
Arnason, B. G. W. & Chelmicka-Szorc, E. Acta Neuropathol. 22, 1–6 (1972).
Yonezawa, T., Ishihara, Y. & Matsuyama, H. J. Neuropathol. exp. Neurol. 27, 453–463 (1968).
Bornstein, M. B. & Appel, S. H. J. Neuropathol. exp. Neurol. 20, 141–157 (1961).
Appel, S. H. & Bornstein, M. B. J. exp. Med. 119, 303–312 (1964).
Leber, R., Boutry, J-M., Vincent, C., Robineaux, R. & Voisin, G. A. J. Immun. 116, 1439–1446 (1976).
Allt, G., Evans, E. M. & Evans, D. H. L. Brain Res. 32, 255–257 (1971).
Chase, M. in Allergic Encephalomyelitis (eds Kies, M. W. & Alvord, E. C. Jr) 348–374 (C. C. Thomas, Springfield, 1959).
Brosnan, C. F., Stoner, G. L., Bloom, B. R. & Wisniewski, H. M. J. Immun. 118, 2103–2110 (1977).
Ballin, R. H. M. & Thomas, P. K. J. neurol Sci. 8, 1–18 (1968).
Lampert, P. W. Lab. Invest. 20, 127–138 (1969).
Schröder, J. M. & Krücke, W. Acta Neuropathol. 14, 261–283 (1970).
Bornstein, M. B. in Tissue Culture: Method and Applications (eds Kruse, P. Jr & Patterson, M. K.) 86–92 (Academic, New York, 1973).
Cochrane, C. G. in Method in Immunology and Immunochemistry (eds Williams, C. A. & Chase M. W.) 5, 169–172 (Academic, New York, 1976).
Whitacre, C. C. & Paterson, P. Y. J. exp. Med. 145, 1405–1410 (1977).
Astrom, K. E., Webster, H. de F. & Arnason, B. G. J. exp. Med. 128, 469–495 (1968).
Jankovic, B. D., Draskoci, M. & Janjie, M. Nature, 207, 428–429 (1965).
Allt, G., Evans, E. M. & Evans, D. H. L. Brain Res. 29, 271–291 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SAIDA, T., SAIDA, K., SILBERBERG, D. et al. Transfer of demyelination by intraneul injection of experimental allergic neuritis serum. Nature 272, 639–641 (1978). https://doi.org/10.1038/272639a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/272639a0
This article is cited by
-
Guillain-Barr� syndrome and serum activities of gamma-Glutamyltransferase and glutamic-pyruvic transaminase
European Archives of Psychiatry and Neurological Sciences (1988)
-
Anti-galactocerebroside antibodiesin human cerebrospinal fluids determined by enzyme-linked immunosorbent assay (ELISA)
Neurochemical Research (1988)
-
An X-ray diffraction study of changes in myelin structure during experimental allergic neuritis
Journal of Neurocytology (1987)
-
Clinical effects of plasmapheresis in acute Guillain-Barré syndrome
The Italian Journal of Neurological Sciences (1986)
-
Blood-nerve barrier studies in experimental allergic neuritis
Acta Neuropathologica (1985)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.