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A new erythroid cell line induced by Rauscher murine leukaemia virus

Nature volume 272, pages 626628 (13 April 1978) | Download Citation

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Abstract

INOCULATION of BALB/c mice with high doses of Rauscher murine leukaemia virus (R-MuLV) results in rapid spleen enlargement due to the accumulation of immature erythroid cells, which subsequently infiltrate other haemopoietic organs1–3. If low doses of virus are injected, the animals overcome the initial erythroid phase of the disease, but usually die later of a lymphatic leukaemia. In newborn C57/Black mice, R-MuLV induces lymphomas4,5, whereas in (C57/B1 X DBA) F1, various types of reticulum cell sarcomas are formed6. Attempts to establish permanent cell lines from these reticulum cell sarcomas were successful, but similar attempts with leukaemic spleen or liver tissue failed repeatedly in BALB/c mice4. Instead, the blast cells matured into haemoglobin-containing cells in vitro7. We report here the induction of tumours in DBA mice using syngeneic transplants of R-MuL V-infected animals in the terminal phase of erythroid leukaemia. From this tumour, a new permanent cell line could be established, which could be induced to synthesise haemoglobin in vitro.

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Affiliations

  1. Department of Experimental Pathology, Medical Faculty, Erasmus University, Rotterdam, The Netherlands

    • N. J. DE BOTH
    • , M. VERMEY
    • , E. VAN 'T HULL
    • , E. KLOOTWIJK-VAN-DIJKE
    • , L. J. L. D. VAN GRIENSVEN
    • , J. N. M. MOL
    •  & T. J. STOOF

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https://doi.org/10.1038/272626a0

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