Article | Published:

DRB-induced premature termination of late adenovirus transcription

Naturevolume 272pages590593 (1978) | Download Citation

Subjects

Abstract

Exposure of HeLa cells to 5, 6-dichloro-1-β-D-ribofura-nosylbenzimidazde (DRB) late in the course of adenovirus 2 infection results in the inhibition of virus-specific RNA synthesis from all parts of the previously identified, very long ( 28 kilobases) late transcriptional unit, except for the first 400–800 nucleotides. It is suggested that DRB acts not as an inhibitor of RNA chain initiation, but rather causes ‘premature’ chain termination close to the promoter. A practical aspect of these findings may be that RNA sequences near promoter sites that are responsible for mRNA formation can be isolated from DRB-treated cells.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Bachenheimer, S. & Darnell, J. E. Proc. natn. Acad. Sci. U.S.A. 72, 4445–4449 (1975).

  2. 2

    Weber, J., Jelinek, W. & Darnell, J. E. Cell 10, 611–616 (1977).

  3. 3

    Goldberg, S., Weber, J. & Darnell, J. E. Cell 10, 617–621 (1977).

  4. 4

    Evans, R., Fraser, N., Ziff, E., Weber, J., Wilson, M. & Darnell, J. E. Cell 12, 733–746 (1977).

  5. 5

    Mosteller, R. D. & Yanofsky, C. J. molec. Biol. 48, 525–531 (1970).

  6. 6

    Egyházi, E. Proc. natn. Acad. Sci. U.S.A. 72, 947–950 (1975).

  7. 7

    Egyházi, E. Nature 262, 319–321 (1976).

  8. 8

    Sehgal, P. B., Derman, E., Molloy, G. R., Tamm, I. & Darnell, J. E. Science 194, 431–433 (1976).

  9. 9

    Tamm, I. & Sehgal, P. B. Adv. Virus Res. 22, 187–258 (1978).

  10. 10

    Flint, J. Cell 10, 153–166 (1977).

  11. 11

    Sehgal, P. B., Darnell, J. E. & Tamm, I. Cell 9, 473–480 (1976).

  12. 12

    Sehgal, P. B., Tamm, I. & Vilček, J. Virology 70, 542–544 (1976).

  13. 13

    Chow, L. T., Roberts, J. M., Lewis, J. B. & Broker, T. R. Cell 11, 819–836 (1977).

  14. 14

    Tibbets, C., Pettersson, U., Johansson, K. & Philipson, L. J. Virol. 13, 370–377 (1974).

  15. 15

    Sharp, P. A., Gallimore, P. H. & Flint, S. J. Cold Spring Harb. Symp. quant. Biol. 39, 457–474 (1974).

  16. 16

    Pettersson, U. & Matthews, M. B. Cell 12, 741–750 (1977).

  17. 17

    Fraser, N. & Ziff, E. J. molec. Biol. (submitted).

  18. 18

    Bertrand, K., Squires, C. & Yanofsky, C. J. molec. Biol. 103, 319–337 (1976).

  19. 19

    Bertrand, K. & Yanofsky, C. J. molec. Biol. 103, 339–349 (1976).

  20. 20

    Roberts, J. W. Proc. natn. Acad. Sci. U.S.A. 72, 3300–3304 (1975).

  21. 21

    Tamm, I. Proc. natn. Acad. Sci. U.S.A. 74, 5011–5015 (1977).

  22. 22

    Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

  23. 23

    Jelinek, W. et al. J. molec. Biol. 75, 515–532 (1973).

  24. 24

    Jelinek, W., Molloy, G., Fernandez-Munoz, R., Salditt, M. & Darnell, J. E. J. molec. Biol. 82, 361–370 (1974).

Download references

Author information

Affiliations

  1. The Rockefeller University, New York, New York, 10021

    • Nigel W. Fraser
    • , Pravinkumar B. Sehgal
    •  & James E. Darnell

Authors

  1. Search for Nigel W. Fraser in:

  2. Search for Pravinkumar B. Sehgal in:

  3. Search for James E. Darnell in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/272590a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.