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DRB-induced premature termination of late adenovirus transcription

Nature 272, 590–593 (1978) | Download Citation

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Abstract

Exposure of HeLa cells to 5, 6-dichloro-1-β-D-ribofura-nosylbenzimidazde (DRB) late in the course of adenovirus 2 infection results in the inhibition of virus-specific RNA synthesis from all parts of the previously identified, very long (∼ 28 kilobases) late transcriptional unit, except for the first ∼ 400–800 nucleotides. It is suggested that DRB acts not as an inhibitor of RNA chain initiation, but rather causes ‘premature’ chain termination close to the promoter. A practical aspect of these findings may be that RNA sequences near promoter sites that are responsible for mRNA formation can be isolated from DRB-treated cells.

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    1. The Rockefeller University, New York, New York, 10021

      • Nigel W. Fraser
      • , Pravinkumar B. Sehgal
      •  & James E. Darnell

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    https://doi.org/10.1038/272590a0

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