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Effect of calcium ionophore Br-X537A on renin synthesis and release in Amphiuma means kidney culture

Nature volume 271, pages 174–176 (1978)Cite this article

Abstract

CALCIUM ions play an important part in stimulus–secretion coupling1, and in particular have been implicated in the control of renin release from kidneys in vitro2. The calcium ionophores A23187 and X537A and its derivatives have been used in several studies of the effect of mobilisation and movement of calcium and other divalent ions on biological systems, including mast cells, pancreatic B cells and the neurohypophysis3–8. X537A and Br-X537A are of interest because they release calcium preferentially from intracellular sites irrespective of the extracellular calcium concentration8,9. In investigating calcium involvement in secretion and the action of ionophores, emphasis has been placed on in vitro studies, where the experimental conditions can be closely controlled. We report here the results of experiments on the effect of Br-X537A on the renin levels in Amphiuma means kidney in organ culture. This system has the advantages of an isolated tissue, which in addition retains its in vivo characteristics for extended periods. We show that Br-X537A has dose-dependent effects on both the synthesis and secretion of renin. These effects apparently represent two quite separate sites of action of the ionophore.

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References

  1. Rubin, R. P. Calcium and the Secretory Process (Plenum, New York, 1974).

    Book  Google Scholar 

  2. Saruta, T. & Matsuki, S. Endocrinol. Japon. 22, 137–140 (1975).

    Article  CAS  Google Scholar 

  3. Scarpa, A., Baldassare, J. & Inesi, G. J. gen. Physiol. 60, 735–749 (1972).

    Article  CAS  Google Scholar 

  4. Foreman, J. C., Mongar, J. L. & Gomperts, B. D. Nature 245, 249–251 (1973).

    Article  ADS  CAS  Google Scholar 

  5. Pressman, B. C. Fedn Proc. 32, 1698–1703 (1973).

    CAS  Google Scholar 

  6. Pressman, B. C. & de Guzman, N. T. Ann. N. Y. Acad. Sci. 227, 380–391 (1974).

    Article  ADS  CAS  Google Scholar 

  7. Somers, G., Devis, G. & Malaisse, W. J. FEBS Lett. 66, 20–22, (1976).

    Article  CAS  Google Scholar 

  8. Nordman, J. J. & Dyball, R. E. J. Nature 225, 414–415 (1975).

    Article  Google Scholar 

  9. Nordmann, J. J. & Currell, G. A. Nature 253, 646–647 (1975).

    Article  ADS  CAS  Google Scholar 

  10. Oparil, S. & Haber, E. New Engl. J. Med. 291, 389–401, 446–457 (1974).

    Article  CAS  Google Scholar 

  11. Veyrat, R., Brunner, H. R., Manning, E. L. & Muller, A. F. J. Urol. Nephrol. Paris 73, 271–275 (1967).

    CAS  PubMed  Google Scholar 

  12. Romero, J. C. & Hoobier, S. W. Am. Heart J. 80, 701–708 (1970).

    Article  CAS  Google Scholar 

  13. Zehr, J. E. & Feigl, E. O. Circulation Res. 32, Suppl. 1, 17–26 (1973).

    Google Scholar 

  14. Hofbauer, K. G., Zschiedrich, H., Hackenthal, E. & Gross, F. Circulation Res. 35, Suppl. 1, 193–201 (1974).

    Google Scholar 

  15. Weinberger, M. H., Aoi, W. & Henry, D. P. Circulation Res. 37, 318–324 (1975).

    Article  CAS  Google Scholar 

  16. Sherwood, J. B., Robinson, S. H., Bassan, L. R., Rosen, S. & Gordon, A. S. Blood 40, 189–197 (1972).

    CAS  PubMed  Google Scholar 

  17. Trowell, O. A. Expl Cell Res. 16, 118–147 (1959).

    Article  CAS  Google Scholar 

  18. Bojeson, E. & Leyssac, P. P. Acta physiol. scand. 65, 20–32 (1965).

    Article  Google Scholar 

  19. MacDougall, J. B. D. & Coupland, R. E. Expl Cell Res. 45, 385–398 (1967).

    Article  CAS  Google Scholar 

  20. Monnickendam, M. A. & Balls, M. Comp. Biochem. Physiol. 50A, 359–363 (1975); J. Cell Sci. 11, 799–813 (1972).

    Article  CAS  Google Scholar 

  21. Cohen, E. L. et al. J. Lab. clin. Med. 77, 1025–1038 (1971).

    CAS  PubMed  Google Scholar 

  22. Haber, E., Koerner, T., Page, L. B., Kliman, B. & Purnode, A. J. clin. Endocr. Metab. 29, 1349–1355 (1969).

    Article  CAS  Google Scholar 

  23. Chambers, E. L., Pressman, B. C. & Rose, B. Biochem. biophys. Res. Commun. 60, 126–132 (1974).

    Article  CAS  Google Scholar 

  24. Fares, R. V. Endocrinology 89, 1057–1063 (1971).

    Article  Google Scholar 

  25. Macleod, R. M. & Fontham, E. H. Endocrinology 86, 863–869 (1970).

    Article  CAS  Google Scholar 

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Author notes

  1. R. T. S. WORLEY

    Present address: Department of Biology, University of Essex, Colchester, UK

Authors and Affiliations

  1. School of Biological Sciences, University of East Anglia, Norwich, UK

    R. T. S. WORLEY & G. T. RICH

  2. Department of Medicine, Norfolk and Norwich Hospital, St. Stephen's Road, Norwich, UK

    J. S. PRYOR

Authors
  1. R. T. S. WORLEY
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  2. G. T. RICH
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  3. J. S. PRYOR
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WORLEY, R., RICH, G. & PRYOR, J. Effect of calcium ionophore Br-X537A on renin synthesis and release in Amphiuma means kidney culture. Nature 271, 174–176 (1978). https://doi.org/10.1038/271174a0

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