Abstract
CELL determination can be described as the progressive restriction in developmental options during embryogenesis1. Mouse embryo cells, in particular, remain undetermined for a relatively long period, and the fate of cells seems to be labile throughout the period of cleavage2–4. Microsurgical analysis of mouse blastocysts, however, has shown that the developmental potential of inner cell mass and trophoblast cells becomes restricted during blastocyst formation, so that neither of these early cell types retains the option of differentiating into the other5–7. We have now investigated whether the primary ectoderm, which forms in the inner cell mass on day 5 of gestation8,9, retains the option of differentiating into endoderm. We found that when ectoderm was isolated from mouse blastocysts by immunosurgery10 it could regenerate an outer layer of endoderm during further culture. The capacity of isolated ectoderm to differentiate into endoderm depended both on the initial mass of the embryo and on the time that the ectoderm was isolated from the inner cell mass.
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PEDERSEN, R., SPINDLE, A. & WILEY, L. Regeneration of endoderm by ectoderm isolated from mouse blastocysts. Nature 270, 435–437 (1977). https://doi.org/10.1038/270435a0
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DOI: https://doi.org/10.1038/270435a0
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