Abstract
IN the course of studies on the regulation of protein synthesis during the activation of human lymphocytes by phytohaemagglutinin (PHA), it became necessary to determine whether an effect which occurred following treatment with actinomycin D (AMD) was secondary to the action of the drug on transcription, or resulted from a direct effect on translation. Using enucleated lymphocytes, we have shown that AMD has no effect on protein synthesis in the absence of the cell nucleus and conclude that AMD interferes with protein synthesis by its effect on transcription of RNA.
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COOPER, H., BRAVERMAN, R. The mechanism by which actinomycin D inhibits protein synthesis in animal cells. Nature 269, 527–529 (1977). https://doi.org/10.1038/269527a0
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DOI: https://doi.org/10.1038/269527a0
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