Productive T7 infection of Escherichia coli F+ cells and anucleate minicells

Abstract

THE development of colivirus T7 is largely independent of host cell functions and dependent on T7-encoded enzymes, for example, T7 RNA polymerase, DNA replication enzymes, and lysozyme1,2. In view of this relative independence, the failure to propagate in Escherichia coli cells carrying an F factor3,4 or in anucleate minicells5 is particularly interesting with respect to a molecular understanding of host–virus interaction. We report here that T7 can, at a low frequency, productively infect both F+ cells and minicells derived from either an F+ or an F E. coli culture. The percentage of T7 permissive cells within an F+ population is dependent on the growth conditions of the culture but does not seem to be directly related to a specific phase of the cell cycle.

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PONTA, H., REEVE, J., PFENNIG-YEH, M. et al. Productive T7 infection of Escherichia coli F+ cells and anucleate minicells. Nature 269, 440–442 (1977). https://doi.org/10.1038/269440a0

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