Translocation of intracellularly stored calcium during the contraction–relaxation cycle in guinea pig taenia coli

Abstract

ALTHOUGH it has been shown that, in vertebrate smooth muscles, cation binding sites are localised in some intracellular structures1–7, and that isolated microsomal fraction can accumulate Ca in the presence of ATP (refs 8–10), the role of the Ca-accumulating structures in the contraction–relaxation cycle is not firmly established. Using potassium pyroantimonate, which is known to penetrate intact cell membrane in the presence of osmium to produce an electron-opaque precipitate with intracellular cations11,12, experiments on a molluscan smooth muscle have provided evidence that Ca localised in some intracellular structures is released into the myoplasm during mechanical activity13,14. We have used this method to investigate the role of the intracellular Ca-accumulating structures in the contraction–relaxation cycle of guinea pig taenia coli. When guinea pig taenia coli was fixed at rest the electron-opaque precipitate containing Ca was observed at or near the plasma membrane. If the fixation was made at the peak of mechanical activity, the precipitate was diffusely distributed in the myoplasm.

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References

  1. 1

    Somlyo, A. V. & Somlyo, A. P. Science 174, 955–958 (1971).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Goodford, P. J. & Wolowyk, M. W. J. Physiol., Lond. 224, 521–535 (1972).

    CAS  Article  Google Scholar 

  3. 3

    Jonas, L. & Zelck, U. Expl Cell Res. 89, 352–358 (1974).

    CAS  Article  Google Scholar 

  4. 4

    Popescu, L. M., Diculescu, I., Zelck, U. & Ionescu, N. Cell Tiss. Res. 154, 357–378 (1974).

    CAS  Article  Google Scholar 

  5. 5

    Somlyo, A. P., Somlyo, A. V., Devine, C. E., Peters, P. D. & Hall, T. A. J. Cell Biol. 61, 723–742 (1974).

    CAS  Article  Google Scholar 

  6. 6

    Popescu, L. M. & Diculescu, I. J. Cell Biol. 67, 911–918 (1975).

    CAS  Article  Google Scholar 

  7. 7

    Heumann, H. G. Cell Tiss. Res. 169, 221–231 (1976).

    CAS  Article  Google Scholar 

  8. 8

    Carsten, M. E. J. gen. Physiol. 53, 414–426 (1969).

    CAS  Article  Google Scholar 

  9. 9

    Batra, S. C. & Daniel, E. E. Comp. Biochem. Physiol. 38A, 285–300 (1971).

    CAS  Article  Google Scholar 

  10. 10

    Hurwitz, L., Fitzpatrick, D. F., Debbas, G. & Landon, E. J. Science 179, 384–386 (1973).

    ADS  CAS  Article  Google Scholar 

  11. 11

    Komnick, H. & Komnick, U. Z. Zellforsch. mikrosk. Anat. 60, 163–203 (1963).

    CAS  Article  Google Scholar 

  12. 12

    Legato, M. J. & Langer, G. A. Cell Biol. 41, 401–423 (1969).

    CAS  Article  Google Scholar 

  13. 13

    Sugi, H. & Atsumi, S. Proc. Japan Acad. 49, 638–642 (1973).

    CAS  Article  Google Scholar 

  14. 14

    Atsumi, S. & Sugi, H. J. Physiol., Lond. 257, 549–560 (1976).

    CAS  Article  Google Scholar 

  15. 15

    Mizuhira, V. Acta histochem. cytochem. 9, 69–87 (1976).

    CAS  Article  Google Scholar 

  16. 16

    Ebashi, S. & Endo, M. Prog. Biophys. molec. Biol. 18, 123–183 (1968).

    CAS  Article  Google Scholar 

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SUGI, H., DAIMON, T. Translocation of intracellularly stored calcium during the contraction–relaxation cycle in guinea pig taenia coli. Nature 269, 436–438 (1977). https://doi.org/10.1038/269436a0

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