ILLUMINATION of chloroplast thylakoids leads to the formation of the so-called high energy state of the membrane1–3. The establishment of this state is accompanied by several structural changes within the membrane, including a conformational change in the coupling factor4, increased accessibility of photosystem II to the chemical probe p-diazonium benzene sulphonate5, and a reduction in the thickness of the partition between stacked thylakoids6. I describe here a rather different type of structural change that has not previously been reported for chloroplast membranes—protein phosphorylation. Like the above changes, protein phosphorylation is a reversible, energy-dependent membrane modification, but it differs from the other changes in that it takes the form of a specific chemical reaction involving certain identifiable chloroplast membrane polypeptides. The most conspicuous of these polypeptides is the light-harvesting chlorophyll a/b binding protein, the most abundant thylakoid polypeptide7.
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Barber, J. The Intact Chloroplast (Elsevier, Amsterdam, 1976).
Govindjee Bioenergetics of Photosynthesis (Academic, New York, 1975).
Anderson, J. M. Biochim. biophys. Acta 416, 191–235 (1975).
Ryrie, I. J. & Jagendorf, A. T. J. biol. Chem. 246, 582–588 (1971).
Giaquinta, R. T. et al. Archs Biochem. Biophys. 162, 200–209 (1974).
Murakami, S. & Packer, L. Biochim. biophys. Acta 180, 420–423 (1969).
Thornber, J. P. A. Rev. Pl. Physiol. 26, 127–158 (1975).
Hall, D. O. Nature new Biol. 235, 125–126 (1972).
Bennett, J. & Milewska, Y. in Genetics and Biogenesis of Chloroplasts and Mitochondria (eds Bücher, T. et al.) 637–640 (Elsevier/North-Holland, Amsterdam, 1976).
O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
Boulter, D. et al. Biol. Rev. 47, 113–175 (1972).
Chu, R. C. L. & Yasumobu, K. T. Biochim. biophys. Acta 89, 148–149 (1964).
Clegg, J. C. S. et al. J. gen. Virol. 32, 413–430 (1976).
Blair, G. E. & Ellis, R. J. Biochim. biophys. Acta 319, 223–234 (1973).
Bennett, J. Phytochemistry 15, 263–265 (1976).
Pai, M. S. et al. Protoplasma 85, 209–218 (1975).
Ralph, R. K. et al. Biochem. J. 130, 901–911 (1972).
Armond, P. A. et al. Archs Biochem. Biophys. 175, 54–63 (1976).
Davis, D. J. et al. Archs Biochem. Biophys. 175, 64–70 (1976).
About this article
Cite this article
BENNETT, J. Phosphorylation of chloroplast membrane polypeptides. Nature 269, 344–346 (1977). https://doi.org/10.1038/269344a0
Comparative analysis of thylakoid protein complexes in state transition mutants nsi and stn7: focus on PSI and LHCII
Photosynthesis Research (2020)
Composition, phosphorylation and dynamic organization of photosynthetic protein complexes in plant thylakoid membrane
Photochemical & Photobiological Sciences (2020)
Historical Records of Australian Science (2019)
Frontiers in Plant Science (2018)
Comparative assessment of chloroplast transcriptional responses highlights conserved and unique patterns across Triticeae members under salt stress
Photosynthesis Research (2018)