Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Identification of chromosomal location of yeast DNA from hybrid plasmid pYelueu10


THERE is strong evidence to suggest that some genes of baker' yeast Saccharomyces cerevisiae can be expressed in Escherichia coli when cloned on plasmid or phage vectors1,2. This conclusion is based on the observation that hybrid plasmids and bacteriophage carrying specific fragments of yeast nuclear DNA can complement certain auxotrophic mutations in the E. coli genome. Complementation alone, however, is not sufficient to prove that the cloned DNA contains a yeast gene equivalent to the defective bacterial gene. One aspect of the identification of any cloned DNA is the identification of its chromosomal origin. We present here a method for the identification of the chromosomal origin of cloned yeast DNA using hybridisation to DNA from yeast strains aneuploid for a single chromosome. Using this method we have confirmed the chromosomal location of the DNA of a yeast hybrid plasmid (pYeleu1O) presumed to contain sequences coding for the leucine biosynthetic enzyme β-isopropylmalate dehydrogenase, the leu2 gene of yeast.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others


  1. Ratzkin, B. & Carbon, J. Proc. natn. Acad. Sci. U.S.A. 74, 487–491 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Struhl, K., Cameron, J. R. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 1471–1475 (1976).

    Article  ADS  CAS  Google Scholar 

  3. Southern, E. J. molec. Biol. 98, 503–518 (1975).

    Article  CAS  PubMed  Google Scholar 

  4. Fink, G. R. & Styles, C. A. Genetics 77, 231–244 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Hawthorne, D. C. Genetics 48, 1727–1729 (1963).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Mortimer, R. K. & Hawthorne, R. C. in The Yeasts (eds Rose, A. H. & Harrison, J.S.) 385–460 (Academic, New York, 1969).

    Google Scholar 

  7. Hicks, J. & Herskowitz, I. Genetics 83, 245–258 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Cryer, D. R., Eccleshall, R. & Marmur, J. Meth. Cell Biol. 12, 39–44 (1975).

    Article  CAS  Google Scholar 

  9. Clewell, D. B. J. Bact. 110, 667–676 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Guerry, P., LeBlanc, D. J. & Falkow, S. J. Bact. 116, 1064–1066 (1973).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1185–1188 (1975).

    Article  ADS  Google Scholar 

  12. Fed. Regist., 41, (no. 131) (1976).

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

HICKS, J., FINK, G. Identification of chromosomal location of yeast DNA from hybrid plasmid pYelueu10. Nature 269, 265–267 (1977).

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI:

This article is cited by


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing