Effect of microtubular antagonists on lymphocyte mitogenesis

Abstract

SEVERAL different classes of mitogens have been discovered1. Stimulation of lymphocytes by all classes of mitogens results in cellular division, but it is not known whether all mitogens act on the cell membrane in identical fashion or result in triggering of the same cytoplasmic messenger. We have investigated the inhibitory effect of microtubule disrupting drugs on lymphocytes transformed by two methods. We report here that colchicine and vinblastine inhibit lectinmediated mitogenesis at low concentrations, while cells stimulated by cell surface oxidation are inhibited only by much higher concentrations of these drugs. It seems that intact microtubules are necessary for lectin-mediated but not for cell surface oxidation-mediated mitogenesis.

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RASMUSSEN, S., DAVIS, R. Effect of microtubular antagonists on lymphocyte mitogenesis. Nature 269, 249–251 (1977). https://doi.org/10.1038/269249a0

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