Primary in vitro sensitisation of human T cells

Abstract

METHODS for the in vitro development of primary immune responses have been crucial for the understanding of mechanisms of cooperation between T and B cells and between T cells and macrophages1–3. Few efforts to date have been reported for primary in vitro sensitisation of human lymphocytes, most of which have involved sensitisation to allo- or xeno-antigens4–6 or plaque-forming responses to sheep red blood cells (SRBC)7,8. Development of killer T cells against HLA-identical9, or autochthonous tumour cells10,11 have usually required coculture with third party allogeneic cells for development of cytotoxicity. Given the ethical constraints on in vivo studies of human T-cell function, and noting the development for the first time of a system for the sensitisation of guinea pig T cells in vitro to a variety of antigens12, we were encouraged to develop an in vitro system for the priming and boosting of human T cells to well defined haptenic determinants without the requirement for a third party cell.

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NEWMAN, W., STONER, G. & BLOOM, B. Primary in vitro sensitisation of human T cells. Nature 269, 151–153 (1977). https://doi.org/10.1038/269151a0

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