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Primary in vitro sensitisation of human T cells

Nature volume 269, pages 151–153 (1977)Cite this article

Abstract

METHODS for the in vitro development of primary immune responses have been crucial for the understanding of mechanisms of cooperation between T and B cells and between T cells and macrophages1–3. Few efforts to date have been reported for primary in vitro sensitisation of human lymphocytes, most of which have involved sensitisation to allo- or xeno-antigens4–6 or plaque-forming responses to sheep red blood cells (SRBC)7,8. Development of killer T cells against HLA-identical9, or autochthonous tumour cells10,11 have usually required coculture with third party allogeneic cells for development of cytotoxicity. Given the ethical constraints on in vivo studies of human T-cell function, and noting the development for the first time of a system for the sensitisation of guinea pig T cells in vitro to a variety of antigens12, we were encouraged to develop an in vitro system for the priming and boosting of human T cells to well defined haptenic determinants without the requirement for a third party cell.

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Author notes

  1. GERALD L. STONER

    Present address: Armauer Hansen Research Institute, PO Box 1005, Addis Ababa, Ethiopia

Authors and Affiliations

  1. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461

    WALTER NEWMAN, GERALD L. STONER & BARRY R. BLOOM

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  1. WALTER NEWMAN
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NEWMAN, W., STONER, G. & BLOOM, B. Primary in vitro sensitisation of human T cells. Nature 269, 151–153 (1977). https://doi.org/10.1038/269151a0

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