Abstract
DNA complementary in sequence to the messenger RNA for pre-proparathyroid hormone was synthesised using reverse transcriptase. In a linked transcription–translation system using RNA polymerase and cell-free extract from wheat germ, the DNA directed the synthesis of a protein identified as pre-proparathyroid hormone by N-terminal Sequencing and by electrophoretic and immunologic criteria.
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Kronenberg, H., Roberts, B., Habener, J. et al. DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription—translation system. Nature 267, 804–807 (1977). https://doi.org/10.1038/267804a0
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DOI: https://doi.org/10.1038/267804a0
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