Abstract
POSTREPLICATION repair of DNA is one pathway for repair of damage inflicted on DNA by physical and chemical agents in the environment1. Postreplication repair can involve error-prone repair of pyrimidine dimers in Escherichia coli2, and as such may be particularly relevant to the understanding of the alteration of initial damage in DNA to a transformed or mutant DNA. The assignment of gene products to essential roles for completion of postreplication repair gap filling after ultraviolet light (UV) damage in E. coli cells3–7 has been complemented by consideration of their respective roles in error-prone repair of UV damage8–11. We have used column chromatography, which separates double-stranded DNA containing single regions from completely double-stranded DNA, to observe the gap filling during postreplication repair of UV damage to DNA. In addition, analysis of E. coli recA or lex mutants demonstrated that the recA+ or the lex+ gene products must control steps exclusive of the initial gap filling process during postreplication repair.
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JOHNSON, R. Gap filling during postreplication repair of DNA in recombination deficient Escherichia coli. Nature 267, 80–81 (1977). https://doi.org/10.1038/267080a0
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DOI: https://doi.org/10.1038/267080a0
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