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Direct detection of depolarisation-induced release of ATP from a synaptosomal preparation


SEVERAL groups have shown that ATP is released from neural tissues during depolarisation, where it or its metabolites might function as neurotransmitters or neuromodulators1. They measured radiolabelled adenosine derivatives in perfusates following depolarisation of their preparations. Recently, Israel et al.2 described a method for continuously and directly detecting the release of ATP from the electric organ of Torpedo marmorata after stimulation of its motor nerve. I have used a modification of this technique to study the depolarisation-induced release of ATP from a purified synaptosomal fraction prepared from rat brain. I report here that depolarisation of synaptosomes elicits a release of ATP which can be directly and continuously detected by this technique.

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WHITE, T. Direct detection of depolarisation-induced release of ATP from a synaptosomal preparation. Nature 267, 67–68 (1977).

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