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Defective dopaminergic regulation of prolactin secretion in a rat pituitary tumour cell line

Nature volume 266, pages 640–641 (1977)Cite this article

Abstract

IT is firmly established that certain rat1,2 and human3,4 pituitary tumours hypersecrete prolactin (PRL). Pituitary PRL secretion is probably under the dual control of hypothalamic prolactin-inhibiting and -releasing factors, and defective secretion in one or both of these factors could produce abnormal PRL secretion3. An alternative explanation for the in vivo PRL secretory defect of certain pituitary tumours is defective reception by the tumour of an appropriate hypothalamic endocrine signal. To test this latter hypothesis, we evaluated the effects of various catecholamines on PRL secretion in a cloned rat pituitary tumour cell line (GH3). Catecholamines are potent inhibitors of PRL secretion5, and it is postulated that the prolactin inhibiting factor may be a catecholamine6,7. We report here that dopamine failed to inhibit PRL secretion from the pituitary tumour cultures, whereas, other catechols added to our normal and pituitary tumour monolayer cultures suppressed prolactin release into the medium. In addition, apomorphine, which is postulated to be a specific dopaminergic agonist8–10, appropriately suppressed PRL secretion in the tumour monolayer cultures. Our results therefore provide evidence for an unusual dopaminergic defect in the GH3 rat pituitary tumour cell line.

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References

  1. Furth, J., Clifton, K. H., Gadsden, E. L. & Buffett, R. I. Cancer Res. 16, 608–615 (1956).

    PubMed  Google Scholar 

  2. Malarkey, W. B. & Daughaday, W. H. Endocrinology 91, 1314–1317 (1972).

    Article  CAS  Google Scholar 

  3. Ganong, W. F. & Martina, L. (eds) Frontiers in Neuroendocrinology 337–374 (Oxford University Press, Oxford, 1973).

  4. Malarkey, W. B. & Johnson, J. C. Arch. intern. Med. 136, 40–44 (1976).

    Article  CAS  Google Scholar 

  5. Birge, C. A., Jacobs, L. S., Hammer, L. T. & Daughaday, W. H. Endocrinology 86, 120–130 (1970).

    Article  CAS  Google Scholar 

  6. MacLeod, R. M. & Lehmeyer, J. E. Endocrinology 94, 1077–1085 (1972).

    Article  Google Scholar 

  7. Sharr, C. J. & Clemens, J. A. Endocrinology 95, 1202–1212 (1974).

    Article  Google Scholar 

  8. Schoenfield, R. & Uretsky, U. Eur. J. Pharmac. 19, 115–118 (1972).

    Article  Google Scholar 

  9. Rekker, R., Engel, D. & Nys, G. J. pharm. Pharmac. 24, 589–591 (1972).

    Article  CAS  Google Scholar 

  10. Smalstig, E. B., Sawyer, B. P. & Clemens, J. A. Endocrinology 95, 123–129 (1974).

    Article  CAS  Google Scholar 

  11. Tashjian, A. H. Jr, Yasumura, Y., Levine, L., Sato, G. H. & Parker, M. L. Endocrinology 82, 342–352 (1968).

    Article  CAS  Google Scholar 

  12. Vale, W., Blackwell, R., Grant, G., Guillemin, R. & Amoss, M. Endocrinology 91, 562–572 (1972).

    Article  CAS  Google Scholar 

  13. Niswender, G. D., Chen, C. L., Midgley, A. R., Meites, J. & Ellis, S. Proc. Soc. exp. Biol. Med. 130, 793–796 (1969).

    Article  CAS  Google Scholar 

  14. Takahara, J., Arimura, A. & Schally, A. Endocrinology 96, 462–465 (1974).

    Article  Google Scholar 

  15. Koch, Y., Lu, K. & Meites, J. Endocrinology 87, 673–675 (1970).

    Article  CAS  Google Scholar 

  16. Janssen, P. et al. Arzneimittel-Forsch. 18, 261–279 (1968).

    CAS  Google Scholar 

  17. Anden, N. E., Butcher, S. G., Carrodi, H., Fuxe & Ungerstedt, V., Eur. J. Pharmac. 11, 303–314 (1970).

    Article  CAS  Google Scholar 

  18. Ojeda, S. R., Harms, P. G. & McCann, S. M. Endocrinology 95, 1694–1703 (1974).

    Article  CAS  Google Scholar 

  19. Dandiya, P. C., Sharma, H. L., Patni, S. K. & Gambhir, R. S. Experientia 15, 1441–1443 (1975).

    Article  Google Scholar 

  20. Malarkey, W. B., Jacobs, L. S. & Daughaday, W. H. New Engl. J. Med. 285, 1160–1163 (1971).

    Article  CAS  Google Scholar 

  21. Van Der Gugten, Sahuleka, P. C., Van Galen, G. H. & Kwa, H. G. J. Endocr. 68, 369–381 (1976).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Ohio State University College of Medicine, Columbus, Ohio, 43210

    W. B. MALARKEY & J. C. GROSHONG

  2. Department of Physiological Chemistry, Ohio State University College of Medicine, Columbus, Ohio, 43210

    G. E. MILO

Authors
  1. W. B. MALARKEY
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  2. J. C. GROSHONG
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  3. G. E. MILO
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MALARKEY, W., GROSHONG, J. & MILO, G. Defective dopaminergic regulation of prolactin secretion in a rat pituitary tumour cell line. Nature 266, 640–641 (1977). https://doi.org/10.1038/266640a0

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