Abstract
Starting from restriction enzyme cleavage sites of known topologies the DNA sequences coding for two thirds of the H2B histone protein, together with some 3′ extracistronic sequences, have been determined for the sea urchin Psammechinus miliaris. This unambiguously identifies this gene and further reveals its 5′–3′ polarity and accurate map position in a cloned histone DNA repeat unit.
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Birnstiel, M., Schaffner, W. & Smith, H. DNA sequences coding for the H2B histone of Psammechinus miliaris. Nature 266, 603–607 (1977). https://doi.org/10.1038/266603a0
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DOI: https://doi.org/10.1038/266603a0
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