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Antibodies to major histocompatibility antigens produced by hybrid cell lines

Naturevolume 266pages550552 (1977) | Download Citation



FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies1,2. One of the most powerful features of this approach is that, by cloning, one may easily derive cell lines synthesising monoclonal antibodies despite using non-purified immunogens. The multiple components of a heterogeneous population of hybrid cells are resolved by cloning techniques. This feature makes the system a very powerful tool in the study of complex antigenic structures. The established cell lines offer the further advantage of unlimited permanent supply of material, and the possibility of worldwide standardisation.

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  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge

    • G. GALFRE
    • , S. C. HOWE
    •  & C. MILSTEIN
  2. On leave of absence from the Department of Medical Genetics, University of Turin, Turin, Italy

    • G. GALFRE
  3. ARC Institute of Animal Physiology, Babraham, Cambridge, UK

    • G. W. BUTCHER
    •  & J. C. HOWARD


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