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Haemoglobin crystals in the midgut of the tick Ornithodorus moubata Murray

Naturevolume 266pages536538 (1977) | Download Citation

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Abstract

Ticks of the Ornithodorus moubata Murray (Ixodoidea, Argasidae) complex, distributed through East Africa, are known as vectors of African relapsing fever1. A laboratory strain of O. moubata porcinus (originally from Tanzania and bred according to the method of Geigy and Herbig1) uses the guinea-pig (Cavia porcellus L.) as experimental host. Guinea pig blood crystallises more easily than that of other hosts2 in the gut of blood-sucking arthropods3. In O. moubata crystals appear 5–10 d after the blood meal, first small (0.015–0.05 mm) and clear red, later up to 0.2 mm in all three directions and dark red. These ‘tick-grown’ crystals may still be present after even more than a year. They possibly serve as nutrient reserve4 since they finally disappear after prolonged starvation. We describe here a study of the formation and structure of these crystals and the changes within these crystals with time. We show the crystals to be composed principally of guinea-pig haemoglobin.

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References

  1. 1

    Geigy, R. & Herbig, A. Acta trop. Suppl. 6, 320–340 (1955).

  2. 2

    Balashov, Y. S. Parazit. Sb. 20, 185–225 (1961).

  3. 3

    Pick, F. Ann. Paras. hum. 40, 1–12 (1965).

  4. 4

    Grandjean, O. & Aeschlimann, A. Acta trop, 30, 193–212 (1973).

  5. 5

    Reichert, E. T. & Brown, A. P. The Differentiation and Specificity of Corresponding Proteins and Other Vital Substances in Relation to Biological Classification and Organic Evolution: The Crystallography of Hemoglobins, 240–242 (Carnegie Institution, Washington, DC, 1909).

  6. 6

    Di-Iorio, E., Winterhalter, K. H., Wilson, K., Rosenmund, A. & Marti, H.-R. Blut 31, 61–68 (1975).

  7. 7

    Zeppezauer, M. Meth. Enzym. 22, 253–266 (1971).

  8. 8

    Matthews, B. W. J. molec. Biol. 33, 491–497 (1968).

  9. 9

    International Tables for X-ray Crystallography 1 (eds Henry, N. M. F. & Lonsdale, K.) 106 (Kynoch, Birmingham, 1969).

  10. 10

    Perutz, M. F., Bolton, W., Diamond, R., Muirhead, H. & Watson, H. C. Nature, 203, 687–690 (1964).

  11. 11

    Weber, K. & Osborn, M. in The Proteins 1 (eds Neurath, H. & Hill, R. L.) 179–223 (Academic, New York, 1975).

  12. 12

    Atlas of Protein Sequence and Structure 5, (ed. Dayhoff, M. O.), D-56, D-64, (National Biochemical Research Foundation, Silver Spring, Maryland, 1972).

  13. 13

    Kleinschmidt, R., Grujic-Injac, B. & Braunitzer, G. Hoppe-Seyler's Z. Physiol. Chem. 354, 687–688 (1973).

  14. 14

    Winterhalter, K. H. in Clinical Biochemistry, Principles and Methods, (eds Curtius, H. C. & Roth, M.) 1305–1322, (Walter de Gruyter, Berlin, 1974).

  15. 15

    Garrick, L. M., Sharma, V. S., McDonald, M. J. & Ranney, H. M. Biochem. J. 149, 245–258 (1975).

  16. 16

    Hecker, H., Diehl, P. A. & Aeschlimann, A. Acta trop. 26, 346–360 (1969).

  17. 17

    Kaufman, S. E. thesis, Univ. British Columbia (1971).

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Author information

Affiliations

  1. Department of Structure Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel

    • J. D. G. SMIT
  2. Institute of Zoology, University of Neuchâtel, 11 rue Emile–Argard, CH-2000, Neuchâtel

    • O. GRANDJEAN
  3. SEM-Laboratory, University of Basel, Bernoullistrasse 32, CH-4056, Basel

    • R. GUGGENHEIM
  4. Friedrich-Miescher-Institute, PO Box 273, CH-4002, Basel, Switzerland

    • K. H. WINTERHALTER

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https://doi.org/10.1038/266536a0

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