Article | Published:

Sequence and structure of D-glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilus

Naturevolume 266pages328333 (1977) | Download Citation

Subjects

Abstract

The glyceraldehyde 3-phosphate dehydogenase holoenzyme of Bacillus stearothermophilus possesses precise 222 symmetry: in this respect it differs from the reported structure of the lobster muscle enzyme. Pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. Three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Harris, J. I. & Waters, M. in The Enzymes XIII, 3rd edn (ed. P. D. Boyer) 1–50 (Academic, New York, 1976).

  2. 2

    Harris, J. I. & Perham, R. N. J. molec. Biol. 13, 876–88 (1965).

  3. 3

    Conway, A. & Koshland, D. E., Jr. Biochemistry 7, 4011–4022 (1968).

  4. 4

    de Vijlider, J. J., Hilvers, A. G., van Lis, J. M. J. & Slater, E. C. Biochem. biophys. Acta 191, 221–228 (1969).

  5. 5

    Seydoux, F., Bernhard, S., Pfenninger, O., Payne, M. & Malhotra, O. P. Biochemistry 12, 4290–4300 (1973).

  6. 6

    Cook, R. A. & Koshland, D. E., Jr. Biochemistry 9, 3337–3342 (1970).

  7. 7

    Kirschner, K. J. molec. Biol. 58, 51–68 (1971).

  8. 8

    Monod, J., Wyman, J. & Changeux, J. P. J. molec. Biol. 12, 88–118 (1965).

  9. 9

    Koshland, D. E., Jr., Némethy, G. & Filmer, D. Biochemistry 5, 365–385 (1966).

  10. 10

    Kirtley, M. E. & Koshland, D. E., Jr. J. biol. Chem. 242, 4192–4205 (1967).

  11. 11

    Bernhard, S. A. & MacQuarrie, R. A. J. molec. Biol. 74, 73–78 (1973).

  12. 12

    Moras, D. et al. J. biol. Chem. 250, 9137–9162 (1975).

  13. 13

    Thierry, J. C., Biesecker, G., Wonacott, A. J., Butler, R. & Sweet, R. M. (in preparation).

  14. 14

    Singleton, R., Kimmel, J. R. & Amelunxen, R. E. J. biol. Chem. 244, 1623–1630 (1969).

  15. 15

    Suzuki, K. & Harris, J. I. FEBS Lett. 13, 217–220 (1971).

  16. 16

    Allen, G. & Harris, J. I. Biochem. J. 151, 747–749 (1975).

  17. 17

    Buehner, M., Ford, G. C., Moras, D., Olsen, K. W. & Rossmann, M. G. Proc. natn. Acad. Sci. U.S.A. 70, 3052–3054 (1973).

  18. 18

    Buehner, M., Ford, G. C., Moras, D., Olsen, K. W. & Rossmann, M. G. J. molec. Biol. 90, 25–40 (1974).

  19. 19

    Hocking, J. D. & Harris, J. I. FEBS Lett. 34, 280–284 (1973).

  20. 20

    Walker, J. E., Bridgen, J. & Harris, J. I. (in preparation).

  21. 21

    Arndt, U. W., Champness, J. N., Phizackerley, R. P. & Wonacott, A. J. J. appl. Crystallogr. 6, 457–463 (1973).

  22. 22

    Bricogne, G. Acta crystallogr. A 30, 395–405 (1974).

  23. 23

    Bricogne, G. Acta crystallogr. A 32, 832–847 (1976).

  24. 24

    Davidson, B. E., Sajgò, M., Noller, H. F. & Harris, J. I. Nature 216, 1181–1185 (1967).

  25. 25

    Harris, J. I., Meriwether, B. P. & Park, J. H. Nature 198, 154–157 (1963).

  26. 26

    Park, J. H., Agnello, C. F., Mathew, E. J. biol. Chem. 241, 769–771 (1966).

  27. 27

    Harris, J. I. & Polgár, L. J. molec. Biol. 14, 630–633 (1965).

  28. 28

    Hill, E., Tsernoglou, D., Webb, L. & Banaszak, L. J. J. molec. Biol. 72, 577–589 (1972).

  29. 29

    Brändén, C. I. et al. Proc. natn. Acad. Sci. U.S.A. 70, 2439–2442 (1973).

  30. 30

    Adams, M. J., Ford, G. C., Liljas, A. & Rossmann, M. G. Biochem. biophys. Res. Commun. 53, 46–51 (1973).

  31. 31

    Rossmann, M. G. et al. J. molec. Biol. 76, 533–537 (1973).

  32. 32

    Olsen, K. W., Moras, D., Rossmann, M. G. & Harris, J. I. J. biol. Chem. 250, 9313–9321 (1975).

  33. 33

    Trentham, D. R. Biochem. J. 122, 59–69 (1970).

  34. 34

    Harrigan, P. J. & Trentham, D. R. Biochem. J. 143, 353–363 (1974).

  35. 35

    Byers, L. O. & Koshland, D. E. Biochemistry 14, 3661–3669 (1975).

  36. 36

    Racker, E. & Krimsky, I. J. biol. Chem. 198, 731–743 (1952).

  37. 37

    Kosower, E. M. J. Am. chem. Soc. 78, 3497–3501 (1956).

  38. 38

    Kosower, E. M. & Klinedinst, D. E., Jr. J. Am. chem. Soc. 78, 3493–3497 (1965).

  39. 39

    Amelunxen, R. E. Biochim. biophys. Acta. 139, 24–32 (1967).

  40. 40

    Amelunxen, R. E., Noelkin, M. & Singleton, R., Jr. Archs Biochem. Biophys. 141, 447–455 (1970).

  41. 41

    Hocking, J. D. & Harris, J. I. in Enzymes and Proteins from Thermophilic microorganisms. (ed. Zuber, H.) 121–134 (Birkhausen Verlag, Basle & Stuttgart, 1976).

  42. 42

    Hocking, J. D. thesis, Univ. Cambridge (1974).

  43. 43

    Perutz, M. F. & Raidt, H. Nature 255, 256–259 (1975).

  44. 44

    Matthews, B. W., Weaver, L. H. & Kester, W. R. J. biol. Chem. 249, 8030–8044 (1974).

  45. 45

    Bricogne, G., Thierry, J. C. & Wonacott, A. J. (in preparation).

  46. 46

    Fletterick, R. J. & Steitz, T. A. Acta crystallogr. A 32, 125–132 (1976).

  47. 47

    Biesecker, A., Thierry, J. C. & Wonacott, A. J. (in preparation).

  48. 48

    Fuller Noel, J. K. & Schumaker, V. W. J. molec. Biol. 68, 523–532 (1972).

  49. 49

    Sloan, D. L. & Velick, S. F. J. biol. Chem. 248, 5419–5423 (1973).

  50. 50

    Smith, G. D. & Schachman, H. K. Biochemistry 12, 3789–3801 (1973).

  51. 51

    Durchschlag, H., Puchwein, G., Kratky, O., Schuster, I. & Eur. J. Biochem. 19, 9–22 (1971).

  52. 52

    Simon, I., Eur. J. Biochem. 30, 184–189 (1972).

  53. 53

    Bornstein, P. Biochemistry 9, 2408–2420 (1970).

  54. 54

    Ommer, G. S., Fontana, A. & Anfinsen, C. B. J. biol. Chem. 245, 1895–1902 (1970).

  55. 55

    Hartley, B. S. Biochem. J. 119, 805–822 (1970).

  56. 56

    Houmard, J. & Drapeau, G. R. Proc. natn. Acad. Sci. U.S.A. 69, 3506–3509 (1972).

Download references

Author information

Author notes

    • G. Biesecker

    Present address: Scripps Clinic & Research Foundation, 476 Prospect Street, La Jolla, California, 92037

    • J. C. Thierry

    Present address: Institut de Chimie, 1 Rue Blaise Pascal, 67008, Strasbourg Cedex, France

Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK

    • G. Biesecker
    • , J. Ieuan Harris
    • , J. C. Thierry
    • , J. E. Walker
    •  & A. J. Wonacott

Authors

  1. Search for G. Biesecker in:

  2. Search for J. Ieuan Harris in:

  3. Search for J. C. Thierry in:

  4. Search for J. E. Walker in:

  5. Search for A. J. Wonacott in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/266328a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.