Malignant transformation of rat embryo fibroblasts by herpes simplex virus types 1 and 2 at suboptimal temperature

Abstract

HERPES SIMPLEX VIRUS (HSV) transforms cultured mammalian cells in three systems, involving (1) ultraviolet-irradiated virus1–4, (2) increased incubation temperature5,6 and (3) temperature-sensitive mutants7,8. Malignant transformation by HSV has been demonstrated for hamster, rat and mouse cells, using three systems4,8–12. We now report a further system, involving the use of a suboptimal temperature. This method is relatively convenient and seems to be applicable to various rat cell lines.

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DARAI, G., BRAUN, R., FLÜGEL, R. et al. Malignant transformation of rat embryo fibroblasts by herpes simplex virus types 1 and 2 at suboptimal temperature. Nature 265, 744–746 (1977). https://doi.org/10.1038/265744a0

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