Vesicular disruption of myelin simulated by exposure of nerve to calcium ionophore

Abstract

EXPERIMENTAL allergic encephalomyelitis (EAE) and experimental allergic neuritis (EAN) are autoimmune demyelinating conditions brought about by sensitisation to central and peripheral myelin extracts, respectively. The lesions are the result of a delayed hypersensitivity-type reaction which can be passively transferred by lymphoid cells but not by sera1–4. Demyelination occurs in areas of cellular infiltrate. This process is mediated in part by mononuclear cells which invade and phagocytise the target myelin tissue. A vesicular transformation of myelin sheaths is known to occur in close proximity to these invading mononuclear cells5–7. The nature of the myelin vesiculation is unclear; however, these changes have been emphasised as a characteristic accompaniment of autoimmune demyelination in experimental animals and in human peripheral nerve disease8. The present study reports the occurrence of the same type of myelin alteration in excised rat peripheral nerve fibres when incubated under conditions which promote a selective influx of calcium ions into the tissues.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Paterson, P. Y. J. exp. Med. 111, 119–136 (1960).

  2. 2

    Stone, S. H. Science 134, 619–620 (1961).

  3. 3

    Åstrom, K. E. & Waksman, B. H. J. path. Bact. 83, 89–106 (1962).

  4. 4

    Levine, S. & Wenk, E. J. J. Immun. 99, 1277–1285 (1967).

  5. 5

    Lampert, P. W. J. Neuropath. exp. Neurol. 24, 371–385 (1965); Acta Neuropath. 9, 99–126 (1967); Lab Invest. 20, 127–138 (1969).

  6. 6

    Ballin, R. H. M. & Thomas, P. K. J. neurol. Sci. 8, 1–18 (1968).

  7. 7

    Wisniewski, H., Prineas, J. & Raine, C. S. Lab invest. 21, 105–118 (1969).

  8. 8

    Dal Canto, M. C., et al., J. neurol. Sci. 24, 313–319 (1975).

  9. 9

    Schlaepfer, W. W. Brain Res. 78, 71–81 (1974).

  10. 10

    Reed, P. W. & Lardy, H. A. J. biol. Chem. 247, 6970–6977 (1972).

  11. 11

    Pfeiffer, D. R., Reed, P. W. & Lardy, H. A. Biochemistry 13, 4007–4014 (1974).

  12. 12

    Deber, C. M. & Pfeiffer, D. R. Biochemistry 15, 132–141 (1976).

  13. 13

    Blank, W. F., Bunge, M. B. & Bunge, R. P. Brain Res. 67, 503–518 (1974).

  14. 14

    Robertson, J. D. J. biophys. biochem. Cytol. 4, 349–364 (1958).

  15. 15

    Hemminki, K. Biochim. biophys. Acta 363, 202–210 (1974).

  16. 16

    Shlatz, L. & Marinetti, G. V. Biochim. biophys. Acta 290, 70–83 (1972).

  17. 17

    Hall, S. M. & Gregson, N. A. J. Cell Sci. 9, 769–789 (1971).

  18. 18

    Webster, H. deF., Spiro, D., Waksman, B. & Adams, R. D. J. Neuropath. exp. Neurol. 20, 5–34 (1961).

  19. 19

    Masurovsky, E. B., Bunge, M. B. & Bunge, R. P. J. Cell Biol. 32, 497–518 (1967).

  20. 20

    Lampert, P. W. & Schochet, S. S. J. Neuropath. exp. Neurol. 27, 527–544 (1968).

  21. 21

    Ballin, R. H. M. & Thomas, P. K. Acta Neuropath. 14, 237–249 (1969).

  22. 22

    Wisniewski, H. & Raine, C. S. Lab. Invest. 25, 73–80 (1971).

  23. 23

    Waksman, B. H. & Namoa, Y. Cell Immun. 21, 161–176 (1976).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

SCHLAEPFER, W. Vesicular disruption of myelin simulated by exposure of nerve to calcium ionophore. Nature 265, 734–736 (1977). https://doi.org/10.1038/265734a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.