Photochemical attachment of cyclic AMP binding protein(s) to the nuclear genome

Abstract

The nature of the cyclic AMP–receptor–nucleus interactions was examined by a novel combination of two photoreactions. A photosensitive derivative of cyclic AMP, N6-butyryl cyclic AMP, was covalently attached to its cytoplasmic receptod by photo-affinity labelling and this receptor complex was photo-crosslinked by the DNA in the rat liver nuclei. The photolytic reactions seemed to be specific since stable links were formed only when substantial noncovalent binding occurred.

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Kallos, J. Photochemical attachment of cyclic AMP binding protein(s) to the nuclear genome. Nature 265, 705–710 (1977). https://doi.org/10.1038/265705a0

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