Abstract
The nature of the cyclic AMP–receptor–nucleus interactions was examined by a novel combination of two photoreactions. A photosensitive derivative of cyclic AMP, N6-butyryl cyclic AMP, was covalently attached to its cytoplasmic receptod by photo-affinity labelling and this receptor complex was photo-crosslinked by the DNA in the rat liver nuclei. The photolytic reactions seemed to be specific since stable links were formed only when substantial noncovalent binding occurred.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jensen, E. V. & DeSombre, E. R. A. Rev. Biochem. 41, 203–230 (1972).
Baxter, J. D. et. al. Proc. natn. Acad. Sci. U.S.A. 69, 1892–1896 (1972).
King, R. J. B. & Mainwaring, W. I. P. Steroid-Cell Interactions (University Park, Baltimore, 1974).
O'Malley, B. W. & Means, A. R. Science 183, 610–620 (1974).
Yamamoto, K. R. & Alberts, B. M. A. Rev. Biochem. 45, 721–746 (1976).
Sutherland, E. W. Science 177, 401–408 (1972).
Robinson, G. A., Butcher, R. W. & Sutherland, E. W. Cyclic AMP (Academic, New York, 1971).
McMahon, D. Science 185, 1012–1021 (1974).
Tomkins, G. M. Science 189, 760–763 (1975).
Kallos, J. Proc. natn. Acad. Sci. U.S.A. (submitted).
Cooperman, B. S. in Aging, Carcinogenesis, and Radiation Biology (ed. Smith, K. C.) 315–346 (Plenum, New York, 1976).
Knowles, J. R. Acct. Chem. Res. 5, 155–160 (1972).
Singer, S. J. Adv. Protein. Chem. 22, 1–54 (1967).
Lin, S. Y. & Riggs, A. D. Proc. natn. Acad. Sci. U.S.A. 71, 947–951 (1974).
Cantor, C. R., Pelligrim, M. & Oen, H. in Ribosomes 573–585 (Cold Spring Harbor Laboratory, 1974).
Markovity, A. Biochim. biophys. Acta 281, 523–534 (1972).
Strniste, G. F. & Smith, D. A. Biochemistry 13, 485–493 (1974).
Shoemaker, H. J. & Schimmel, P. R. J. molec. Biol. 84, 503–513 (1974).
Weintraub, H. Cold Spring Harb. Symp. quant. Biol. 38, 247–256 (1974).
Cuthrow, C. E., Rasmussen, H., Brunswisk, D. J. & Cooperman, B. S. Proc. natn. Acad. Sci. U.S.A. 70, 3344–3346 (1973).
Filler, R. & Litwack, G. Biochem. biophys. Res. Commun. 52, 159–167 (1973).
Turro, N. J. et al. Acct. Chem. Res. 5, 92–101 (1972).
Tao, M. Meth. Enzym. 38, 155–160 (1974).
Ruoho, A. E., Kiefer, H., Roeder, P. E. & Singer, S. J. Proc. natn. Acad. Sci. U.S.A. 70, 2567–2571 (1973).
Johnson, E. D., Hadden, J. W., Inoue, A. & Allfrey, V. G. Biochemistry 14, 3873–3884 (1975).
Jungmann, R. A., Hiestand, P. C. & Schweppe, J. S. Endocrinolology 94, 168–183 (1974).
Yamamoto, K. R. & Alberts, B. M. J. biol. Chem. 249, 7076–7086 (1974).
Keely, S. J., Corbin, J. D. & Park, C. R. Proc. natn. Acad. Sci. U.S.A. 72, 1501–1504 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kallos, J. Photochemical attachment of cyclic AMP binding protein(s) to the nuclear genome. Nature 265, 705–710 (1977). https://doi.org/10.1038/265705a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/265705a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.