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Nucleotide sequence of bacteriophage φX174 DNA

Abstract

A DNA sequence for the genome of bacteriophage φX174 of approximately 5,375 nucleotides has been determined using the rapid and simple ‘plus and minus’ method. The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Two pairs of genes are coded by the same region of DNA using different reading frames.

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References

  1. Sanger, F. & Coulson, A. R. J. molec. Biol. 94, 441–448 (1975).

    CAS  Article  PubMed  Google Scholar 

  2. Benbow, R. M., Hutchison, C. A. III, Fabricant, J. D. & Sinsheimer, R. L. J. Virol. 7, 549–558 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Benbow, R. M., Zuccarelli, A. J., Davis, G. C. & Sinshiemer, R. L. J. Virol. 13, 898–907 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Denhardt, D. T. CRC Crit. Rev. Microbiol. 4, 161–222 (1975).

    CAS  Article  PubMed  Google Scholar 

  5. Hall, J. B. & Sinsheimer, R. L. J. molec. Biol. 6, 115–127 (1963).

    CAS  Article  PubMed  Google Scholar 

  6. Ling, V. Proc. natn. Acad. Sci. U.S.A. 69, 742–746 (1972).

    ADS  CAS  Article  Google Scholar 

  7. Harbers, B., Delaney, A. D., Harbers, K. & Spencer, J. H. Biochemistry 15, 407–414 (1976).

    CAS  Article  PubMed  Google Scholar 

  8. Burton, K. & Petersen, G. B. Biochem. J. 75, 17–27 (1960).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. Chadwell, H. A. Thesis, University of Cambridge (1974).

  10. Sadowski, P. D. & Bakyta, I. J. biol. Chem. 247, 405–412 (1972).

    CAS  PubMed  Google Scholar 

  11. Ling, V. FEBS Lett. 19, 50–54 (1971).

    ADS  CAS  Article  PubMed  Google Scholar 

  12. Ziff, E. B., Sedat, J. W. & Galibert, F. Nature new Biol. 241, 34–37 (1973).

    CAS  Article  PubMed  Google Scholar 

  13. Galibert, F., Sedat, J. W. & Ziff, E. B. J. molec. Biol. 87, 377–407 (1974).

    CAS  Article  PubMed  Google Scholar 

  14. Robertson, H. D., Barrell, B. G., Weith, H. L. & Donelson, J. E. Nature new Biol. 241, 38–40 (1973).

    CAS  Article  PubMed  Google Scholar 

  15. Air, G. M. & Bridgen, J. Nature new Biol. 241, 40–41 (1973).

    CAS  Article  PubMed  Google Scholar 

  16. Sanger, F., Donelson, J. E., Coulson, A. R., Kössel, H. & Fischer, D. Proc. natn. Acad. Sci. U.S.A. 70, 1209–1213 (1973).

    ADS  CAS  Article  Google Scholar 

  17. Schott, H. Makromolek. Chem. 175, 1683–1693 (1974).

    ADS  CAS  Article  Google Scholar 

  18. Donelson, J. E., Barrell, B. G., Weith, H. L., Kössel, H. & Schott, H. Eur. J. Biochem. 58, 383–395 (1975).

    CAS  Article  PubMed  Google Scholar 

  19. Blackburn, E. H. J. molec. Biol. 93, 367–374 (1975).

    CAS  Article  PubMed  Google Scholar 

  20. Blackburn, E. H. J. molec. Biol. 107, 417–432 (1976).

    CAS  Article  PubMed  Google Scholar 

  21. Sedat, J. W., Ziff, E. B. & Galibert, F. J. molec. Biol. 107, 391–416 (1976).

    CAS  Article  PubMed  Google Scholar 

  22. Air, G. M. J. molec. Biol. 107, 433–444 (1976).

    CAS  Article  PubMed  Google Scholar 

  23. Air, G. M. et al. J. molec. Biol. 107, 445–458 (1976).

    CAS  Article  PubMed  Google Scholar 

  24. Air, G. M., Blackburn, E. H., Sanger, F. & Coulson, A. R. J. molec. Biol. 96, 703–719 (1975).

    CAS  Article  PubMed  Google Scholar 

  25. Lee, A. S. & Sinsheimer, R. L. Proc. natn. Acad. Sci. U.S.A. 71, 2882–2886 (1974).

    ADS  CAS  Article  Google Scholar 

  26. Hayashi, M. N. & Hayashi, M. J. Virol. 14, 1142–1152 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Vereijken, J. M., van Mansfeld, A. D. M., Baas, P. D. & Jansz, H. S. Virology 68, 221–233 (1975).

    CAS  Article  PubMed  Google Scholar 

  28. Jeppesen, P. G. N., Sanders, L. & Slocombe, P. M. Nucl. Acids Res. 3, 1323–1339 (1976).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  29. Sato, S., Hutchison, C. A. III & Harris, J. I. Proc. natn. Acad. Sci. U.S.A. (in the press).

  30. Brown, N. L. & Smith, M. FEBS Lett. 65, 284–287 (1976).

    CAS  Article  PubMed  Google Scholar 

  31. Fiddes, J. C. J. molec. Biol. 107, 1–24 (1976).

    CAS  Article  PubMed  Google Scholar 

  32. Air, G. M., Sanger, F. & Coulson, A. R. J. molec. Biol. 108, 519–533 (1976).

    CAS  Article  PubMed  Google Scholar 

  33. Barrell, B. G., Air, G. M. & Hutchison, C. A. III Nature 264, 34–41 (1976).

    ADS  CAS  Article  PubMed  Google Scholar 

  34. Smith, L. H. & Sinsheimer, R. L. J. molec. Biol. 103, 699–735 (1976).

    CAS  Article  PubMed  Google Scholar 

  35. Grohmann, K., Smith, L. H. & Sinsheimer, R. L. Biochemistry 14, 1951–1955 (1975).

    CAS  Article  Google Scholar 

  36. Axelrod, N. J. molec. Biol. 108, 753–779 (1976).

    CAS  Article  PubMed  Google Scholar 

  37. Hayashi, M., Fujimura, F. K. & Hayashi, M. Proc. natn. Acad. Sci. U.S.A. 73, 3519–3523 (1976).

    ADS  CAS  Article  Google Scholar 

  38. Pribnow, D. Proc. natn. Acad. Sci. U.S.A. 72, 784–788 (1975).

    ADS  CAS  Article  Google Scholar 

  39. Rosenberg, M., de Crombrugghe, B. & Musso, R. Proc. natn. Acad. Sci. U.S.A. 73, 717–721 (1976).

    ADS  CAS  Article  Google Scholar 

  40. Bertrand, K. et al. Science 189, 22–26 (1975).

    ADS  CAS  Article  PubMed  Google Scholar 

  41. Sugimoto, K., Sugisaki, H., Okamoto, T. & Takanami, M. J. molec. Biol. (in the press).

  42. Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).

    ADS  CAS  Article  Google Scholar 

  43. Steitz, J. A. & Jakes, K. Proc. natn. Acad. Sci. U.S.A. 72, 4734–4738 (1975).

    ADS  CAS  Article  Google Scholar 

  44. Henry, T. J. & Knippers, R. Proc. natn. Acad. Sci. U.S.A. 71, 1549–1553 (1974).

    ADS  CAS  Article  Google Scholar 

  45. Linney, E. & Hayashi, M. Nature 249, 345–348 (1974).

    ADS  CAS  Article  PubMed  Google Scholar 

  46. Baas, P. D., Jansz, H. S. & Sinsheimer, R. L. J. molec. Biol. 102, 633–656 (1976).

    CAS  Article  PubMed  Google Scholar 

  47. Eisenberg, S., Harbers, B., Hours, C. & Denhardt, D. T. J. molec. Biol. 99, 107–123 (1975).

    CAS  Article  PubMed  Google Scholar 

  48. Subramanian, K. N., Dhar, R. & Weissman, S. M. J. biol. Chem. (in the press).

  49. Ravetch, J. V., Model, P. & Robertson, H. D. Nature 265, 698–702 (1977).

    ADS  CAS  Article  PubMed  Google Scholar 

  50. Smith, M. et al. (submitted to Nature).

  51. Funk, F. & Sinsheimer, R. L. J. Virol. 6, 12–19 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  52. Baas, P. D., van Heusden, G. P. H., Vereijken, J. M., Weisbeek, P. J. & Jansz, H. S. Nucl. Acids Res. 3, 1947–1960 (1976).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  53. Platt, T. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 72, 2399–2403 (1975).

    ADS  CAS  Article  Google Scholar 

  54. Weisbeek, P. J., Vereijken, J. M., Baas, P. D., Jansz, H. S. & Van Arkel, G. A. Virology 72, 61–71 (1976).

    CAS  Article  PubMed  Google Scholar 

  55. Jazwinski, S. M., Lindberg, A. A. & Kornberg, A. Virology 66, 283–293 (1975).

    CAS  Article  PubMed  Google Scholar 

  56. Kornberg, A. DNA Synthesis (W. H. Freeman, San Francisco, 1974).

    Google Scholar 

  57. Chen, C. Y., Hutchison, C. A. III & Edgell, M. H. Nature new Biol. 243 233–236 (1973).

    CAS  Article  PubMed  Google Scholar 

  58. van Mansfeld, A. D. M., Vereijken, J. M. & Jansz, H. S. Nucl. Acids Res. 3, 2827–2843 (1976).

    CAS  Article  Google Scholar 

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Sanger, F., Air, G., Barrell, B. et al. Nucleotide sequence of bacteriophage φX174 DNA. Nature 265, 687–695 (1977). https://doi.org/10.1038/265687a0

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  • DOI: https://doi.org/10.1038/265687a0

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