Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes

Abstract

The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Rheinwald, J. G. & Green, H. Cell 6, 317–330 (1975).

  2. 2

    Rheinwald, J. G. & Green, H. Cell 6, 331–344 (1975).

  3. 3

    Sun, T.-T. & Green, H. Cell 9, 511–521 (1976).

  4. 4

    Hayflick, L. Expl Cell Res. 37, 614–636 (1965).

  5. 5

    Savage, C. R., Inagami, T. & Cohen, S. J. biol. Chem. 247, 7612–7621 (1972).

  6. 6

    Cohen, S. J. biol. Chem. 237, 1555–1562 (1962).

  7. 7

    Cohen, S. & Elliott, G. A. J. Invest. Dermatol. 40, 1–5 (1963).

  8. 8

    Cohen, S. Devl. Biol. 12, 394–407 (1965).

  9. 9

    Turkington, R. W. Expl Cell Res. 57, 79–85 (1969).

  10. 10

    Bertsch, S. & Marks, F. Nature 251, 517–519 (1974).

  11. 11

    Starkey, R. H., Cohen, S. & Orth, D. N. Science 189, 800–802 (1975).

  12. 12

    Armelin, H. A. Proc. natn. Acad. Sci. U.S.A. 70, 2702–2706 (1973).

  13. 13

    Hollenberg, M. D. & Cuatrecasas, P. Proc. natn. Acad. Sci. U.S.A. 70, 2964–2968 (1973).

  14. 14

    Cohen, S., Carpenter, G. & Lembach, K. J. in Adv. Metabolic Disorders (eds Lust, R. & Hall, K.) 8, 265–284 (Academic Press, New York, 1975).

  15. 15

    Westermark, B. Biochem. biophys. Res. Commun. 69, 304–310 (1976).

  16. 16

    Hollenberg, M. D. Archs Biochem. Biophys. 171, 371–377 (1975).

  17. 17

    Byyny, R. L., Orth, D. N., Cohen, S. & Doyne, E. S. Endocrinol. 95, 776–782 (1974).

  18. 18

    Savage, Jr, C. R. & Cohen, S. J. biol. Chem. 247, 7609–7611 (1972).

  19. 19

    Gospodarowicz, D., Weseman, J., Moran, J. S. & Lindstrom, J. J. Cell. Biol. 70, 395–405 (1976).

  20. 20

    Hayflick, L. & Moorhead, P. S. Expl Cell Res. 25, 585–621 (1961).

  21. 21

    Fulder, S. J. & Holliday, R. Cell 6, 67–73 (1975).

  22. 22

    Todaro, G. J. & Green, H. Proc. Soc. exp. Biol. Med. 116, 688–692 (1964).

  23. 23

    Macieira-Coelho, A. Experientia 22, 390–391 (1966).

  24. 24

    Cristofalo, V. J. in Aging in Cell and Tissue Culture (eds Holecková, & Cristofalo, V. J.), 83–119 (Plenum Press, New York, 1970).

  25. 25

    Martin, S., Ogburn, C. & Sprague, C. in Adv. Exp. Med. & Biol. (eds Cristofalo, V. J., Roberts, J. & Adelman, R. C.) 61, 163–193 (Plenum, New York, 1975).

  26. 26

    Lajtha, L. G. in Regulation of Hematopoiesis (ed. A. S. Gordon) 1, 111–131 (Appleton-Century-Crofts, New York, 1970).

  27. 27

    Siminovitch, L., Till, J. E. & McCulloch, E. A. J. Cell comp. Physiol. 64, 23–32 (1964).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rheinwald, J., Green, H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 265, 421–424 (1977). https://doi.org/10.1038/265421a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.