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Molecular weights of antihaemophilic factor and von Willebrand factor proteins in human plasma


HUMAN factor VIII contains at least two biological activities, antihaemophilic factor (AHF) and von Willebrand factor (VWF). When highly purified, factor VIII is estimated to have a molecular weight of over 1.12 × 106, as determined by gel chromatography or sedimentation equilibrium centrifugation1–4. Some investigators2–4 consider factor VIII a single glycoprotein with a number of covalently linked subunits. Since factor VIII can be dissociated in certain conditions, others5–7 consider it to be a two-molecule complex consisting of a multi-subunit high molecular weight protein (> 106) with VWF activity that acts as a carrier molecule for a lower molecular weight (2.4 × 105) subunit with AHF activity. A third model suggests that both AHF and VWF are high molecular weight, separate proteins consisting of disulphide linked subunits8,9.

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NEWMAN, J., HARRIS, R. & JOHNSON, A. Molecular weights of antihaemophilic factor and von Willebrand factor proteins in human plasma. Nature 263, 612–613 (1976).

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