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Formation of stable crystalline enzyme–substrate intermediates at sub-zero temperatures

Nature volume 263, pages 294–297 (1976)Cite this article

Abstract

Sub-zero temperatures can be used to trap intermediates in enzyme-catalysed reactions using suitable cryosolvents. The feasibility of obtaining such intermediates in the crystalline state for X-ray diffraction studies has been demonstrated with several proteases, using specific substrates and optimal pH.

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Authors and Affiliations

  1. Division of Natural Sciences, University of California, Santa Cruz, California, 95064

    Anthony L. Fink & Ahmed I. Ahmed

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  1. Anthony L. Fink
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  2. Ahmed I. Ahmed
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Fink, A., Ahmed, A. Formation of stable crystalline enzyme–substrate intermediates at sub-zero temperatures. Nature 263, 294–297 (1976). https://doi.org/10.1038/263294a0

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