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Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants

Nature volume 262, pages 795–797 (1976)Cite this article

Abstract

CELL separation techniques, the possibility of recognising different markers by immunofluorescence and functional tests, have subdivided cells once thought to be a homogeneous group into different subclasses. This development has been most prominent for the so-called mononuclear cells. A difficulty inherent in the classification of such subclasses (of, for example, mononuclear cells) is that it is often almost impossible to determine precisely what percentage of the cells belongs to a certain subclass, and whether the sum of the subclasses is equal to, lower than or even higher than 100% of the total of the cells analysed. A typical recent example was the identification of the “null” or K cell1,2. Such analysis is greatly facilitated if we analyse a cell suspension stained with two or more different fluorescent dyes visible at the same time. This is feasible.

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Authors and Affiliations

  1. Department of Immunohaematology, University Hospital Leiden, Leiden

    J. J. VAN ROOD & A. VAN LEEUWEN

  2. Department of Histochemistry and Cytochemistry, University of Leiden, Leiden, The Netherlands

    J. S. PLOEM

Authors
  1. J. J. VAN ROOD
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  2. A. VAN LEEUWEN
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  3. J. S. PLOEM
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VAN ROOD, J., VAN LEEUWEN, A. & PLOEM, J. Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants. Nature 262, 795–797 (1976). https://doi.org/10.1038/262795a0

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