Nonsense suppressors of Saccharomyces cerevisiae can be generated by mutation of the tyrosine tRNA anticodon

Abstract

The yeast amber suppressor, SUP5-a, was previously shown to cause the insertion of tyrosine at the sites of UAG nonsense codons. Nucleotide sequencing established that this SUP5-a suppressor specifies a mutant tyrosine transfer RNA (tRNA) which has the anticodon CΨA instead of the normal GΨA, an alteration identical to that found in the tyrosine-inserting amber suppressor from Escherichia coli.

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