THERE is no definitive teleological interpretation of the involvement of H–2 determinants in the recognition by mouse cytolytic T cells of various non-H–2 antigens1,2. While it is generally believed that T-cell immunity against non-H–2 structures (determined, for example, by infectious agents) may benefit from the known special reactivity of T cells against non-self H–2, other interpretations have been proposed, such as the possibility that H–2 is necessary for cytolysis at a postrecognition stage as the “weak point” of the target-cell surface3. A prediction of this hypothesis is that target cells devoid of H–2 should be resistant to T-cell-mediated cytolysis. We have tested this prediction using male germinal cells, embryonal carcinoma and virus-transformed testicular cells as targets, in the presence of the lectin concanavalin A (con A). Con A binding apparently by-passes4 the normal recognition system, so that lack of recognition of H–2 on H–2-less target cells would not be a limiting factor for cytolysis. We found that H–2-less embryonal carcinoma cell lines, including one with barely detectable levels of F9 antigen, were sensitive to lysis, strongly suggesting that at a postrecognition stage H–2 is not required for T-cell-mediated cytolysis.
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Shearer, G. M., Eur. J. Immun., 4, 527–533 (1974).
Zinkernagel, R. M., and Doherty, P. C., Nature, 248, 701–702 (1974).
Forman, J., J. exp. Med., 142, 403–418 (1975).
Forman, J., and Möller, G., J. exp. Med., 138, 672–685 (1973).
Vojtiskova, M., and Pokorna, Z., Folia Biol., 18, 1–9 (1972).
Fellous, M., Erikson, R., Dubois, P., and Gachelin, G., Folia Biol. (in the press).
Artzt, K., and Jacob, F., Transplantation, 17, 633–634 (1974).
Forman, J., and Vitetta, E. S., Proc. natn. Acad. Sci. U.S.A, 72, 3661–3665 (1975).
Artzt, K., et al., Proc. natn. Acad. Sci. U.S.A., 70, 2988–2992 (1973).
Artzt, K., and Bennett, D., Nature, 256, 545–547 (1975).
Nicolas, J. F., Avner, P., Gaillard, J., Jacob, H., and Jacob, F., Cancer Res. (in the press).
Bevan, M. J., and Cohn, M., J. Immun., 114, 559–565 (1975).
Bonavida, B., and Bradley, T. P., Transplantation, 21, 94–102 (1976).
Röllinghoff, M., Pfizenmeier, K., Trostmann, H., and Wagner, H., Eur. J. Immun., 5, 560–564 (1975).
Golstein, P., and Smith, E. T., Contemp. Topics Immunobiol., 7 (in the press).
Hammerberg, C., and Klein, J., Nature, 258, 296–299 (1975).
Mayer, M. M., Proc. natn. Acad. Sci. U.S.A., 69, 2954–2958 (1972).
Golstein, P., Nature, 252, 81–83 (1974).
About this article
European Journal of Immunology (1985)
Selective binding of concanavalin A to target cell major histocompatibility antigens is required to induce nonspecific conjugation and lysis by cytolytic T lymphocytes in lectin-dependent cytotoxicity
Cellular Immunology (1984)
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