Human cytomegalovirus infection of WI-38 cells stimulates mitochondrial DNA synthesis


HUMAN cytomegalovirus (HCMY) infection has been shown to lead to the stimulation of host cell DNA and RNA synthesis in both permissive and non-permissive cells1–3. This effect on DNA synthesis occurred at about 12–16 h after infection, and preceded synthesis of viral DNA by 36 h. The host cell DNA species which are stimulated have not been characterised. We show here that much of the stimulation of DNA synthesis was due to an increased rate of mitochondrial DNA synthesis and that ethidium bromide inhibited HMCV replication.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    St Jeor, S., Albrecht, T. B., Funk, F. D., and Rapp, F., J. Virol., 13, 353 (1974).

    Article  CAS  Google Scholar 

  2. 2

    Tanaka, S., Furukawa, T., and Plotkin, S. A., J. Virol., 15, 297 (1975).

    Article  CAS  Google Scholar 

  3. 3

    Furukawa, T., Tanaka, S., and Plotkin, S. A., Proc. Soc. exp. Biol. Med., 148, 211 (1975).

    Article  CAS  Google Scholar 

  4. 4

    Levine, A. J., Proc. natn. Acad. Sci. U.S.A., 68, 717 (1971).

    ADS  Article  CAS  Google Scholar 

  5. 5

    Neuhard, J., Randerath, E., and Randerath, K., Analyt. Biochem., 13, 211 (1965).

    Article  CAS  Google Scholar 

  6. 6

    Plummer, G., Goodheart, C. R., Henson, D., and Bowling, C. P., Virology, 39, 134 (1969).

    Article  CAS  Google Scholar 

  7. 7

    Iwasaki, Y., Furukawa, T., Plotkin, S. A., and Koprowski, H., Arch. Gesamte Virusforsch., 40, 311 (1973).

    Article  CAS  Google Scholar 

  8. 8

    Smith, J. D., and Harven, E. de, J. Virol., 14, 945 (1974).

    Article  CAS  Google Scholar 

  9. 9

    Wiebel, F., and Baserga, R., J. Cell Physiol., 74, 191 (1974).

    Article  Google Scholar 

  10. 10

    Vesco, C., and Basilico, C., Nature, 229, 336 (1971).

    ADS  Article  CAS  Google Scholar 

  11. 11

    Radsak, K., and Albring, M., J. gen. Virol., 25, 457 (1974).

    Article  CAS  Google Scholar 

  12. 12

    Neuberger, A., and Tatum, E. L., Frontiers of Biology, II (North Holland, 1969).

    Google Scholar 

  13. 13

    Furukawa, T., Fioretti, A., and Plotkin, S. A., J. Virol., 11, 991 (1973).

    Article  CAS  Google Scholar 

  14. 14

    Vesco, C., and Penman, S., Biochem. biophys. Res. Commun., 35, 249 (1969).

    Article  CAS  Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

FURUKAWA, T., SAKUMA, S. & PLOTKIN, S. Human cytomegalovirus infection of WI-38 cells stimulates mitochondrial DNA synthesis. Nature 262, 414–416 (1976).

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.