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Synaptic transmission and effects of temperature at the squid giant synapse

Abstract

THERE have been several reports on the effects of temperature on transmitter release at synaptic junctions1–6. Although all reports agree that synaptic transmission is affected by temperature, the relationships are often complex and may differ from one preparation to another. The preparations studied, however, have the disadvantage that the relationship of presynaptic events (such as spike amplitude) to postsynaptic response cannot be investigated. It is possible to study this relationship at the squid giant synapse where intracellular recording can be obtained from both the presynaptic terminal and the postsynaptic axon. The excitatory postsynaptic potential (e.p.s.p.) at the squid synapse usually generates an action potential in the postsynaptic axon. To study the e.p.s.p., and hence transmitter release, without the postsynaptic action potential, tetrodotoxin has often been used 7–9; this has not permitted study of the characteristics of the presynaptic spike in relation to the e.p.s.p. In a recent investigation on transmission at the squid giant synapse, we found that lowering the temperature of the preparation abolished activation of the postsynaptic action potential by the e.p.s.p., while the presynaptic spike was still generated. This observation enabled us to investigate the relationship between the presynaptic spike and the e.p.s.p. in various experimental conditions10 and we report here the effects of changing temperature.

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References

  1. 1

    Eccles, J. C., Katz, B., and Kuffler, S. W., J. Neurophysiol., 4, 362–387 (1941).

    Article  Google Scholar 

  2. 2

    Boyd, I. A., and Martin, A. R., J. Physiol., Lond., 132, 61–73 (1956).

    CAS  Article  Google Scholar 

  3. 3

    Takeuchi, N., Jap. J. Physiol., 8, 390–404 (1958).

    Google Scholar 

  4. 4

    Hofmann, W. W., Parsons, R. L., and Feigen, F. A., Am. J. Physiol., 211, 135–140 (1960).

    Article  Google Scholar 

  5. 5

    Hubbard, J. I., Jones, S. F., and Landau, E. M., J. Physiol., Lond., 216, 591–609 (1971).

    CAS  Article  Google Scholar 

  6. 6

    Ward, D., Crowley, W. J., and Johns, T. R., Am. J. Physiol., 222, 216–219 (1972).

    CAS  Article  Google Scholar 

  7. 7

    Bloedel, J., Gage, P. W., Llinas, R., and Quastel, D. M. J., Nature, 212, 49–50 (1966).

    ADS  CAS  Article  Google Scholar 

  8. 8

    Katz, B., and Miledi, R., J. Physiol. Lond., 192, 407–436 (1967).

    CAS  Article  Google Scholar 

  9. 9

    Kusano, K., Livengood, D. R., and Werman, R., J. gen. Physiol., 50, 2579–2601 (1967).

    CAS  Article  Google Scholar 

  10. 10

    Erulkar, S. D., and Weight, F. F., Society for Neuroscience, Fourth Annual Meeting Abstr., 202 (1974).

  11. 11

    Hodgkin, A. L., and Katz, B., J. Physiol., Lond., 109, 240–249 (1949).

    CAS  Article  Google Scholar 

  12. 12

    Lester, H. A., Nature, 227, 493–496 (1970).

    ADS  CAS  Article  Google Scholar 

  13. 13

    Hagiwara, S., and Tasaki, I., J. Physiol., Lond., 143, 114–137 (1958).

    CAS  Article  Google Scholar 

  14. 14

    Takeuchi, A., and Takeuchi, N., J. gen. Physiol., 45, 1181–1193 (1962).

    CAS  Article  Google Scholar 

  15. 15

    Miledi, R., and Slater, C. R., J. Physiol., Lond., 184, 473–498 (1966).

    CAS  Article  Google Scholar 

Download references

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WEIGHT, F., ERULKAR, S. Synaptic transmission and effects of temperature at the squid giant synapse. Nature 261, 720–722 (1976). https://doi.org/10.1038/261720a0

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