Abstract
DIFFERENTIAL staining of sister chromatids can be demonstrated with Hoechst 33258 fluorochrome1 and with Giemsa after special pretreatment2, even if 5-bromodeoxyuridine (BUdR) is present during the first S phase only3. These techniques permit the use of the phenomenon of the sister chromatid exchange (SCE). The average spontaneous rate of 10–14 SCEs per metaphase seems to be fairly constant in all cultures from several species4,5. A variety of mutagenic factors (that is, ultraviolet light and alkylating agents) produce very high frequencies of SCE even at concentrations far below the level necessary for the induction of chromosomal breakage, whereas others, like X rays or certain chemicals, hardly influence the rate of SCE6. Because of the high turnover of BrdU in the liver and the physiologically available thymidine, differential chromatid staining has so far only been produced in in vitro systems (cell cultures) and in the chicken embryo.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Latt, S., Proc. natn. Acad. Sci. U.S.A., 70, 3395–3399 (1973).
Perry, P., and Wolff, S., Nature, 251, 156–158 (1974).
Korenberg, J. R., and Freedlender, E. F., Chromosoma, 48, 355–360 (1974).
Wolff, S., and Perry, P., Chromosoma, 48, 341–353 (1974).
Galloway, S. M., and Evans, H. J., Cytogenet. Cell Genet., 15, 17–29 (1975).
Perry, P., and Evans, H. J., Nature, 258, 121–125 (1975).
Bloom, S. E., and Hsu, T. C., Chromosoma, 51, 261–267 (1975).
Epplen, J. T., Siebers, J.-W., and Vogel, W., Cytogenet. Cell Genet., 15, 177–185 (1975).
Savage, K. R., Nature, 258, 103–104 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
VOGEL, W., BAUKNECHT, T. Differential chromatid staining by in vivo treatment as a mutagenicity test system. Nature 260, 448–449 (1976). https://doi.org/10.1038/260448a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/260448a0
This article is cited by
-
Correlation between level of sister chromatid exchanges and chromosomal aberrations induced by chemical mutagensin vivo
Bulletin of Experimental Biology and Medicine (1983)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
