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Structure of the lactose operator

Abstract

THE lac operon has long been an important model system for developing an understanding of the control of gene expression. Since the early studies of Jacob and Monod1 on the lac operon, much work has been devoted towards determining the mode of interaction between repressor and operator. Although it has been shown clearly that repressor physically binds operator2,3, the details of this interaction remain unknown. We report here results of experiments designed to investigate further the structure of the operator and its interaction with the repressor.

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References

  1. Jacob, F., and Monod, J., J. molec. Biol, 3, 318–356 (1961).

    Article  CAS  PubMed  Google Scholar 

  2. Gilbert, W., and Muller-Hill, B., Proc. natn. Acad. Sci. U.S.A., 58, 2415–2421 (1967).

    Article  ADS  CAS  Google Scholar 

  3. Ptashne, M., Nature, 214, 232–234 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Gilbert, W., and Maxam, A., Proc. natn. Acad. Sci. U.S.A., 70, 3581–3584 (1973).

    Article  ADS  CAS  Google Scholar 

  5. Bernardi, G., Adv. Enzymol., 31, 1–49 (1968).

    CAS  PubMed  Google Scholar 

  6. Gierer, A., Nature, 212, 1480–1481 (1966).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Sobell, H., Proc. natn. Acad. Sci. U.S.A., 69, 2483–2487 (1972).

    Article  ADS  CAS  Google Scholar 

  8. Dickson, R., Abelson, J., Barnes, W., and Reznikoff, W., Science, 187, 27–35 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Dykes, G., Bambara, R., Marians, K., and Wu, R., Nucl. Acids Res., 2, 327–345 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Allet, B., and Bukhari, A., J. molec. Biol., 92, 529–540 (1975).

    Article  CAS  PubMed  Google Scholar 

  11. Gilbert, W., Gralla, J., Majors, J., and Maxam, A., Protein–Ligand Interactions, 193–210 (Walter de Gruyter, Berlin, 1975).

    Google Scholar 

  12. Wang, J. C., Barkley, M. D., and Bourgeois, S., Nature, 251, 247–249 (1974).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Richmond, T. J., and Steitz, T. A., Fedn Proc., 34, Abs. 554 (1975).

    Google Scholar 

  14. Chan, H., and Wells, R., Nature, 252, 205–209 (1974).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Rushizky, G. W., Shaternikov, V. A., Mosejko, J. H., and Sober, H. A., Biochemistry, 14, 4221–4226 (1975).

    Article  CAS  PubMed  Google Scholar 

  16. Shenk, T., Rhodes, C., Rigby, P., and Berg, P., Proc. natn. Acad. Sci. U.S.A., 72, 989–993 (1975).

    Article  ADS  CAS  Google Scholar 

  17. Vogt, V., Eur. J. Biochem., 33, 192–200 (1973).

    Article  CAS  PubMed  Google Scholar 

  18. Wu, R., Padmanabhan, R., and Bambara, R., Meth. Enzymol., 29 E, 231–253 (1974).

    Article  CAS  Google Scholar 

  19. Kelly, T. J., and Smith, H. O., J. molec. Biol., 51, 393–409 (1970).

    Article  CAS  PubMed  Google Scholar 

  20. Platt, T., Files, J. G., and Weber, K., J. biol. Chem., 248, 110–121 (1973).

    CAS  PubMed  Google Scholar 

  21. Donelson, J., and Wu, R., J. biol. Chem., 247, 4661–4668 (1972).

    CAS  PubMed  Google Scholar 

  22. Ardelt, W., and Laskowski, M., Sr, Biochem. biophys. Res. Commun., 44, 1205–1211 (1971).

    Article  CAS  PubMed  Google Scholar 

  23. Wu, R., Jay, E., and Roychoudhury, R., in Methods in Cancer Research, 12, (Academic, New York, 1976).

    Google Scholar 

  24. Kössel, H., and Roychoudhury, R., Eur. J. Biochem., 22, 271–276 (1971).

    Article  PubMed  Google Scholar 

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MARIANS, K., WU, R. Structure of the lactose operator. Nature 260, 360–363 (1976). https://doi.org/10.1038/260360a0

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